Exploitation of a new flagellatropic phage of Erwinia for positive selection of bacterial mutants attenuated in plant virulence: towards phage therapy
Exploitation of a new flagellatropic phage of Erwinia for positive selection of bacterial mutants attenuated in plant virulence: towards phage therapy
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DOI：
10.1111/j.1365-2672.2009.04462.x
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发表时间：
2010-02-01
影响因子：
4
通讯作者：
Salmond, G. P. C.
中科院分区：
文献类型：
--
作者：
Evans, T. J.;Trauner, A.;Salmond, G. P. C.
目的：分离和鉴定植物病原体胡萝卜软腐欧文氏菌的新型噬菌体。方法和结果：在马铃薯腐烂细菌种Eca上分离到一种新的致鞭毛噬菌体，并利用电子显微镜和限制性内切酶分析对其进行了表征。这种噬菌体被命名为Theta AT 1，有一个二十面体的头部和一个长的可收缩的尾巴;它属于肌尾病毒科。部分测序显示存在与大肠杆菌噬菌体T4、T7和Mu同源的基因。Eca的噬菌体抗性转座子突变体被分离出来，并在体外研究了一些毒力相关的表型;只有运动被发现受到影响。在体内块茎腐烂试验表明，这些突变体的毒力减弱，大概是因为感染是无法从最初的网站infection.Conclusions传播：Eca鞭毛可以作为一个受体Theta AT 1 infections，和抗性突变体的运动性和毒力defictions.Significance和影响的研究：Theta AT 1是第一个报告的鞭毛营养噬菌体发现感染Eca，并使进一步研究这种经济上重要的植物病原体的毒力。
Aims:To isolate and characterize novel bacteriophages for the phytopathogen, Erwinia carotovora ssp. atroseptica (Eca), and to isolate phage-resistant mutants attenuated in virulence.Methods and Results:A novel flagellatropic phage was isolated on the potato-rotting bacterial species, Eca, and characterized using electron microscopy and restriction analysis. The phage, named Theta AT1, has an icosahedral head and a long, contractile tail; it belongs to the Myoviridae family. Partial sequencing revealed the presence of genes with homology to those of coliphages T4, T7 and Mu. Phage-resistant transposon mutants of Eca were isolated and studied in vitro for a number of virulence-related phenotypes; only motility was found to be affected. In vivo tuber rotting assays showed that these mutants were attenuated in virulence, presumably because the infection is unable to spread from the initial site of inoculation.Conclusions:The Eca flagellum can act as a receptor for Theta AT1 infection, and resistant mutants are enriched for motility and virulence defects.Significance and Impact of the Study:Theta AT1 is the first reported flagellatropic phage found to infect Eca and has enabled further study of the virulence of this economically important phytopathogen.