VARIABILITY IN COLLAGEN AND FIBRONECTIN SYNTHESIS BY SCLERODERMA FIBROBLASTS IN PRIMARY CULTURE
VARIABILITY IN COLLAGEN AND FIBRONECTIN SYNTHESIS BY SCLERODERMA FIBROBLASTS IN PRIMARY CULTURE
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DOI：
10.1111/1523-1747.ep12520933
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发表时间：
1981-01-01
影响因子：
6.5
通讯作者：
TIMPL, R
中科院分区：
文献类型：
--
作者：
FLEISCHMAJER, R;PERLISH, JS;TIMPL, R
从乳头状、网状和皮下获得的成纤维细胞的原代培养物。通过代谢标记和放射免疫测定分析硬皮病皮肤层蛋白质合成。与未受影响患者的细胞相比，几种培养物显示总蛋白胶原、纤连蛋白和III型前胶原的产生增加10-20倍。大多数增加在网状和s.c.层次。对于来自其他患者的细胞，在某些外植体中或仅在某些产物中合成增加。观察到的异质性可以代表疾病、研究的细胞或获得细胞时疾病状态的异质性。
Primary cultures of fibroblasts obtained from the papillary, reticular and s.c. layer of scleroderma skin were analyzed for protein synthesis by metabolic labeling and radioimmunoassay. Several cultures showed a 10-20-fold increase in the production of total protein collagen, fibronectin and type III procollagen as compared to cells from unaffected patients. Most increases were noted in the reticular and s.c. layers. With cells from other patients, increased synthesis was fond in some of the explants or for only some of the products. The heterogeneity observed could represent heterogeneity in the disease, in the cells studied or in the state of the disease at the time the cells were obtained.