Quality evaluation of snow lotus (Saussurea): quantitative chemical analysis and antioxidant activity assessment
Quality evaluation of snow lotus (Saussurea): quantitative chemical analysis and antioxidant activity assessment
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DOI：
10.1007/s00299-010-0919-4
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发表时间：
2010-12-01
影响因子：
6.2
通讯作者：
Ma, Fengshan
中科院分区：
文献类型：
--
作者：
Qiu, Jian;Xue, Xiaofeng;Ma, Fengshan
雪莲是一种常用的药用植物，具有很高的药理价值。为了保护这些濒危植物，建立了离体繁殖和细胞培养，以满足日益增长的市场需求。对3种常用药用植物的酚类成分、抗氧化活性、总酚含量（TPC）和总黄酮含量（TFC）、离体繁殖系和细胞培养物进行了研究，以确定其药理价值。定量分析结果表明，不同物种间及同一物种在不同生境中的酚类物质含量差异较大。由此可以推断，酚类化合物受遗传背景和环境条件的影响。抗氧化活性与几种酚类物质/TPC/TFC显著相关，表明酚类物质是雪莲抗氧化活性的主要贡献者，对雪莲品质评价具有重要意义。根据酚类物质、总酚含量、总黄酮含量和抗氧化活性的高低，天山雪莲野生种的质量顺序为天山雪莲>水母雪莲>绵毛雪莲。对于鼠伤寒沙门氏从产地看，其质量为日喀则>拉萨>那曲。对于离体繁殖的植物，成熟的植物可以是一个可靠的替代野生植物，酚类化合物的动态是至关重要的，这种一次结实的物种的质量控制。我们提供了野生植物和细胞培养物之间的质量比较的第一份报告。发展细胞培养的优势，从野生收集的植物作为替代品进行了讨论。
Snow lotus is commonly used as a medicinal plant and has great pharmacological value. To protect these endangered plants, in vitro propagation and cell cultures have been established in order to meet the growing market demand. The phenolic composition, antioxidant activities, total phenolic content (TPC) and total flavonoid content (TFC) from three most commonly used species, in vitro propagated lines and the cell cultures were investigated to qualify their pharmacological value. Quantitative analysis showed that the phenolics varied greatly among different species and the same species at different habitats. From this it can be inferred that the phenolics were influenced by genetic background and the environmental conditions. Significant correlations were observed between the antioxidant activity and several phenolics/TPC/TFC, suggesting that the phenolics are a major contributor of the antioxidant activity and are important for quality evaluation of snow lotus. Based on the abundance of phenolics, TPC, TFC and antioxidant activity, the order of the quality for wild species would be Saussurea involucrata > Saussurea medusa > Saussurea gossypiphora. For S. medusa, its quality judged by origin would be Shigatse > Lhasa > Nagqu. For in vitro propagated plants, the matured plants could be a reliable substitute for wild plants, and the dynamics of phenolics is critical for quality control of this monocarpic species. We provide the first report of quality comparison between the wild plants and the cell cultures. The advantages of developing cell cultures as alternatives for plants collected from the wild are discussed.