Co-culture update: Creating an embryotrophic environment in vitro
Co-culture update: Creating an embryotrophic environment in vitro
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DOI：
10.1055/s-2007-1016276
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发表时间：
1998-01-01
期刊：
影响因子：
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通讯作者：
Conway-Myers, BA
中科院分区：
文献类型：
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作者：
Conway-Myers, BA
随着不孕症治疗的发展和技术的发展，以提高受精率和怀孕率，很明显，在体外环境中培养配子/胚胎是不完美的，当与体内对应。这对于那些想要孩子的夫妇来说是一个严重的问题，但他们受到不成功的怀孕尝试和模仿体内环境的技术的限制，这种技术可以增强配子相互作用和胚胎发育。为了开始将配子/胚胎的体内和体外微环境相关联，并认识到输卵管作为卵母细胞横向进入子宫的通道的潜力，将输卵管细胞沿着配子和胚胎的共培养引入体外受精群体。本报告试图汇编那些试图通过细胞与配子和/或胚胎共培养来改善体外培养条件以提高妊娠率的研究和结果。
As infertility treatments evolve and techniques are developed to improve the fertilization and pregnancy rates, it is clear that the in vitro environment in which the gametes/embryos are cultured is less than perfect when compared to the in vivo counterpart. This becomes a serious problem for couples who seek to have children but are limited by unsuccessful attempts to become pregnant and a technology that is behind in mimicking the in vivo environment that would enhance gamete interaction and embryo development. In an attempt to begin to correlate the in vivo and in vitro microenvironment of the gametes/embryos and to recognize the potential of the fallopian tube as move then a highway for oocytes to transverse in their pathway to the uterus, coculturing of tubal cells along with gametes and embryos was introduced to the in vitro fertilization community. This report is an attempt to compile the research and results of those attempting to improve pregnancy rates by improving in vitro culture conditions via co-culture of cells with, gametes and/or embryos.