Manual acupuncture needle stimulation of the rat hindlimb activates groups I, II, III and IV single afferent nerve fibers in the dorsal spinal roots
Manual acupuncture needle stimulation of the rat hindlimb activates groups I, II, III and IV single afferent nerve fibers in the dorsal spinal roots
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DOI：
10.2170/jjphysiol.r2120
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发表时间：
2005-06-01
期刊：
影响因子：
--
通讯作者：
Aikawa, Y
中科院分区：
文献类型：
--
作者：
Kagitani, F;Uchida, S;Aikawa, Y
本实验采用大鼠单单位神经记录技术，旨在确定在第4或第5腰椎节段（L4或L5）的背根中哪些特定的传入神经纤维群（组I，II，III和IV）在手动针刺刺激过程中被激活。将直径为300-340 μ m的针灸针插入后肢足三里穴（ST 36）周围的皮肤和下层肌肉中，在椎板切除术后，在靠近进入脊髓的入口处切断L4和L5脊神经的背根，并游离解剖以记录单一传入神经活动针刺刺激激活的单个传入纤维被识别为刺激过程中和单脉冲电刺激坐骨神经引起的电诱发动作电位过程中相同形状的放电尖峰。通过电诱发动作电位潜伏期计算传入纤维的传导速度。有意记录所有动物的共计35个单位，以包括所有4个传入纤维组。在没有刺激的情况下，单位自发沉默，而所有单位对同侧手动旋转针灸针作出反应。所有35个单位的传导速度范围在0.8和86.0 m/s之间，因此属于I-IV组纤维。I、II、III和IV组的平均传导速度分别为57.9 m/s（n = 13）、42.9 m/s（n = 11）、10.3 m/s（n = 6）和1.2 m/s（n = 5）。针刺刺激Ⅰ、Ⅱ、Ⅲ、Ⅳ组传入纤维时的平均放电频率分别为7.4Hz、6.2Hz、4.7Hz和0.4Hz。IV组传入纤维的放电率显著低于I、II和III组传入纤维。结论：手针刺激大鼠后肢激活了属于所有四组传入神经纤维。提示手针刺激所激活的四组躯体传入，在动作电位传递到中枢神经系统时，会引起不同的效应。
Using single unit nerve recording techniques in rats, the present experiment aimed to determine which specific population of afferent nerve fibers (groups I, II, III and IV) in the dorsal roots at the 4th or 5th lumbar segments (L4 or L5) are activated during manual acupuncture needle stimulation. An acupuncture needle 300-340 mu m in diameter was inserted into the skin and underling muscles around the Zusanli acupoint (ST36) area in the hindlimbs, and was manually rotated right and left at a frequency of about 1 Hz for 1 min. The dorsal root of the L4 and L5 spinal nerve was cut close to the entrance into the spinal cord after laminectomy and dissected free to record unitary afferent nerve activity. A single afferent fiber activated by acupuncture stimulation was identified by the identical shape of the discharge spikes during stimulation and during electrically evoked action potentials induced by single pulse electrical stimulation of the sciatic nerve. The conduction velocity of the afferent fiber was calculated by the latency of the electrically evoked action potential. A total of 35 units were intentionally recorded from all animals in order to include all 4 afferent fiber groups. Units were spontaneously silent in the absence of stimulation, while all units responded to ipsilateral manual rotation of the acupuncture needle. The conduction velocity of all 35 units ranged between 0.8 and 86.0 m/s, thus belonging to groups I-IV fibers. Mean conduction velocity of groups I, II, III and IV were 57.9 m/s (n = 13), 42.9 m/s (n = 11), 10.3 m/s (n = 6) and 1.2 m/s (n = 5), respectively. Mean discharge rates during acupuncture stimulation of groups I, II, III and IV afferents were 7.4 Hz, 6.2 Hz, 4.7 Hz and 0.4 Hz, respectively. Discharge rates of group IV afferent fibers were significantly lower than those of groups I, II and III afferents. It was concluded that manual acupuncture needle stimulation to the hindlimbs activated afferent nerve fibers belonging to all four groups of afferents in rats. It is suggested that all four groups of somatic afferents activated by manual acupuncture stimulation will elicit various effects when action potentials are delivered to the central nervous system.