Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus.
Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus.
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DOI：
10.4238/2013.november.7.7
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发表时间：
2013-11
期刊：
影响因子：
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通讯作者：
Lina Sun;Hongsheng Yang;Muyan Chen;D. Xu
中科院分区：
文献类型：
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作者：
Lina Sun;Hongsheng Yang;Muyan Chen;D. Xu
许多发育基因在再生过程中起着重要作用。本研究利用cDNA末端快速扩增技术（RACE）和聚合酶链反应（PCR）技术克隆了仿刺参（Apostichopus）的两个发育相关基因Wnt 6和Hox 6。Wnt 6基因全长1140 bp，开放阅读框为1029 bp，编码342个氨基酸。Wnt 6蛋白含有一个22个氨基酸的信号肽和一个274个氨基酸的成熟肽。该基因全长1312 bp，开放阅读框966 bp，编码321个氨基酸。其推导的氨基酸序列包括一个同源结构域（60个氨基酸）和三个组成复杂度较低的结构域。比较和系统发育分析表明，Wnt 6和Hox 6在A.日本海胆与海胆的亲缘关系最近。实时荧光定量PCR分析表明，Wnt 6和Hox 6在肠再生过程中表达明显上调，提示它们参与了海参肠再生过程。
Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein contained a 22-amino acid signal peptide and a 274-amino acid mature peptide. The Hox6 (AjHBOX6) full-length cDNA contained 1312 bp with a 966-bp open reading frame encoding 321 amino acids. Its deduced amino acid sequence included one homeodomain (60 amino acids) and three domains with low compositional complexity. Comparison and phylogenetic analysis revealed that Wnt6 and Hox6 in A. japonicus had the nearest relationship to those in sea urchins. Further expression analysis by real-time PCR showed that Wnt6 and Hox6 were apparently upregulated during intestine regeneration, which suggested that they were involved in the intestinal regeneration of sea cucumbers.