Immunohistochemical study of oral epithelial sheets cultured on amniotic membrane for oral mucosal reconstruction
Immunohistochemical study of oral epithelial sheets cultured on amniotic membrane for oral mucosal reconstruction
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DOI：
10.3233/bme-2010-0613
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发表时间：
2010-01-01
影响因子：
1
通讯作者：
Kanamura, Narisato
中科院分区：
文献类型：
--
作者：
Amemiya, Takeshi;Nakamura, Takahiro;Kanamura, Narisato
我们用免疫组织化学方法评价了生长在羊膜上的口腔上皮细胞是否可以作为一种有效的修复口腔黏膜缺损的生物材料。白兔口腔黏膜上皮细胞在AM载体上与3T3成纤维细胞共培养2~3周。用自体口腔上皮片移植修复兔口腔黏膜缺损。然后对口腔上皮片和重建的组织进行组织学和免疫组织化学检查。培养2-3周后，兔口腔黏膜上皮细胞在AM上形成5.7层复层免疫组织化学显示它们表达角蛋白4/13、整合素α6、α5链和III型胶原，而不表达角蛋白1/10。移植片附着于粘膜缺损处，AM碎片从移植区消失。免疫组织化学方法显示重建上皮中的粘膜和基底膜成分的特性。实验结果表明，AM培养的口腔上皮片可诱导出粘膜样分化，并附着于黏膜缺损处。因此，AM培养的口腔上皮片可能是一种有用的口腔黏膜重建生物材料。
We immunohistochemically evaluated whether oral epithelial cells grown on amniotic membrane (AM) would be an effective biomaterial for reconstructing oral mucosal defects. Oral mucosal epithelial cells from albino rabbits were grown for 2-3 weeks on an AM carrier in a co-culture with 3T3 fibroblasts. The rabbits' oral mucosal defects were reconstructed by autologous transplantation of the oral epithelial sheets. The oral epithelial sheets and reconstructed tissues were then examined histologically and immunohistochemically. After 2-3 weeks of culture, the rabbit oral mucosal epithelial cells developed 5 7 layers of stratification on the AM. Immunohistochemistry revealed that they expressed keratins 4/13, integrin alpha 6, alpha 5 chain and collagen type III, but not keratins 1/10. The transplanted sheets attached to the mucosal defects, and AM fragments disappeared from the transplant area. Immunohistochemical patterns revealed properties of the mucous membrane and basement membrane components in the reconstructed epithelia. The results of this experiment showed that the AM-cultured oral epithelial sheets resulted in mucosa-like differentiation, and adhered to the mucosal defects. Therefore, AM-cultured oral epithelial sheets might be a useful biomaterial for oral mucosal reconstruction.