Labelling and tracking of human mesenchymal stromal cells in preclinical studies and large animal models of degenerative diseases.
Labelling and tracking of human mesenchymal stromal cells in preclinical studies and large animal models of degenerative diseases.
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DOI：
10.2174/1574888x09666140521144559
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发表时间：
2014-08
影响因子：
2.7
通讯作者：
M. Vaegler;J. Maerz;B. Amend;Luis Arenas da Silva;J. Mannheim;K. Fuchs;S. Will;K. Sievert;A. Stenzl;M. Hart;W. Aicher
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文献类型：
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作者：
M. Vaegler;J. Maerz;B. Amend;Luis Arenas da Silva;J. Mannheim;K. Fuchs;S. Will;K. Sievert;A. Stenzl;M. Hart;W. Aicher
干细胞治疗的成功在不同的医学学科中都有报道，包括血液学、风湿学、骨科外科、创伤学等。目前，4000多项干细胞临床试验已经完成或正在进行，其中378项研究或正在研究间充质基质细胞(MSCs)。大多数使用干细胞或祖细胞的临床试验，包括造血干细胞和MSCs，都是针对免疫系统的。然而，基于MSCs的治疗方法被越来越多地应用于治疗以下症状：常驻干细胞原位失效或组织或结构的功能障碍与免疫细胞或炎症无关，而与机械或代谢应激、衰老、发育或获得性畸形等原因有关。为了进一步开发干细胞疗法作为外科和其他医学专业的安全有效的治疗方法，需要详细探讨移植到临床前模型中的骨髓间充质干细胞的行为及其对应用部位的影响。根据所采用的临床前模型，对活体动物中标记的干细胞的跟踪对于探索MSC介导的组织再生的机制和动力学具有巨大的差异。在这里，我们回顾了(临床前)标记人MSCs的关键方法，用于在小动物和大动物模型中进行短期和长期观察。
Success of stem cell therapies were reported in different medical disciplines, including haematology, rheumatology, orthopaedic surgery, traumatology, and others. Currently, more than 4000 clinical trials using stem cells have been completed or are underway, among which 378 investigated or are at present investigating mesenchymal stromal cells (MSCs). The majority of clinical trials using stem- or progenitor- cells, including hematopoietic stem cells and MSCs, target the immune system. However, therapies based on MSCs are increasingly implemented to treat symptoms in which failure of the resident stem cells in situ, or malfunction of tissues or structures are not associated with immune cells or inflammation, but instead are associated with mechanical or metabolic stress, ageing, developmental or acquired malformations, and other causes. To proceed further in the development of stem cell therapies as a safe and effective treatment for surgical and other medical specialities, the behaviour of MSCs implanted in preclinical models and their impact on the site of application need to be explored in detail. Depending on the pre-clinical model employed, tracking of labelled stem cells in live animals makes an enormous difference for exploration of the mechanisms and kinetics involved in MSC-mediated tissue regeneration. Here we review (pre-)clinically applicable key methods to label human MSCs for short and long-term observations in small and large animal models.