Conditional production of a functional fish growth hormone in the transgenic line of Nannochloropsis oculata (Eustigmatophyceae)
Conditional production of a functional fish growth hormone in the transgenic line of Nannochloropsis oculata (Eustigmatophyceae)
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DOI：
10.1111/j.1529-8817.2008.00508.x
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发表时间：
2008-06-01
影响因子：
2.9
通讯作者：
Tsai, Huai-Jen
中科院分区：
文献类型：
--
作者：
Chen, Hsin Liang;Li, Si Shen;Tsai, Huai-Jen
将含有由热激蛋白70 A启动子和RUBISCO SSU 2启动子驱动的鱼类生长激素（GH）cDNA的质粒phr-YPGHc转入海洋微藻Nannochloropsis oculata（Droop）D. J.通过电穿孔的Hibiscus。获得了4个转基因克隆，其中转移的phr-YPGHc整合到基因组中，并稳定存在至少到第50代。当我们通过热激处理这些转基因微藻时，异源鱼GH的产生量为0.42至0.27 μ g。mL（-1）。我们将卤虫与野生型和转基因N. oculata培养6 h，然后将这些微藻处理的卤虫饲喂红罗非鱼幼虫。喂食后，喂食用转基因微藻孵育的卤虫的幼虫的生长更大（即，统计显著性：P < 0.05）：体重增加316%对104%，体长增加217%对146%。因此，N. oculata能够生产功能性GH，我们建议它可能是一个很好的生物反应器材料。
Plasmid phr-YPGHc, containing the fish growth hormone (GH) cDNA driven by a heat shock protein 70A promoter and a RUBISCO SSU 2 promoter, was transferred into the protoplast of marine microalga Nannochloropsis oculata (Droop) D. J. Hibberd by electroporation. Four transgenic clones were obtained in which the transferred phr-YPGHc was integrated into the genome and existed stably at least until the 50th generation. When we treated these transgenic microalgae by heat shock, the heterologous fish GH was produced in the amount of 0.42 to 0.27 mu g . mL(-1) from the 50 mL of medium. We incubated artemia with the wildtype and transgenic N. oculata for 6 h and then fed these microalgae-treated artemia to red-tilapia larvae. After feeding, the growth of larvae that were fed artemia incubated with transgenic microalgae was greater (i.e., statistically significant: P < 0.05) than that of larvae that were fed artemia incubated with nontransgenic microalgae: 316% versus 104% in weight gain, and 217% versus 146% in body length increase, respectively. Therefore, the N. oculata enables production of functional GH, and we propose that it might be an excellent bioreactor material.