Unusual regulation of a leaderless operon involved in the catabolism of dimethylsulfoniopropionate in Rhodobacter sphaeroides.
Unusual regulation of a leaderless operon involved in the catabolism of dimethylsulfoniopropionate in Rhodobacter sphaeroides.
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DOI：
10.1371/journal.pone.0015972
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发表时间：
2011-01-07
期刊：
影响因子：
3.7
通讯作者：
Johnston AW
中科院分区：
文献类型：
--
作者：
Sullivan MJ;Curson AR;Shearer N;Todd JD;Green RT;Johnston AW
球形红杆菌菌株2.4.1是一种被广泛研究的细菌，最近已显示其将丰富的海洋抗应激分子二甲基磺基丙酸酯（DMSP）裂解成丙烯酸酯和气态二甲基硫醚。它通过使用由dddL编码的裂解酶来实现，dddL是三基因操纵子acuR-acuI-dddL的启动子远端基因。当细胞与底物DMSP预生长时，操纵子的转录增强，但这种诱导是间接的，并且需要DMSP转化为产物丙烯酸酯，真正的共诱导剂。这种调节是由启动子近端基因acuR的产物介导的，acuR是TetR家族中的转录调节因子。AcuR在不存在丙烯酸酯的情况下抑制操纵子，但这通过共诱导剂的存在而缓解。另一个不寻常的调控特征是acuR-acuI-dddL mRNA转录物是无前导的，因此acuR缺乏Shine-Dalgarno核糖体结合位点和5′-UTR，并且与下游基因相比以较低的水平翻译。这种调控单元可能在细菌中相当广泛，因为其他几个分类学上不同的谱系具有相邻的acuR样和acuI样基因;这些操纵子也没有5′前导序列或核糖体结合位点，并且它们的预测顺式作用调控序列类似于R.类球孢菌
Rhodobacter sphaeroides strain 2.4.1 is a widely studied bacterium that has recently been shown to cleave the abundant marine anti-stress molecule dimethylsulfoniopropionate (DMSP) into acrylate plus gaseous dimethyl sulfide. It does so by using a lyase encoded by dddL, the promoter-distal gene of a three-gene operon, acuR-acuI-dddL. Transcription of the operon was enhanced when cells were pre-grown with the substrate DMSP, but this induction is indirect, and requires the conversion of DMSP to the product acrylate, the bona fide co-inducer. This regulation is mediated by the product of the promoter-proximal gene acuR, a transcriptional regulator in the TetR family. AcuR represses the operon in the absence of acrylate, but this is relieved by the presence of the co-inducer. Another unusual regulatory feature is that the acuR-acuI-dddL mRNA transcript is leaderless, such that acuR lacks a Shine-Dalgarno ribosomal binding site and 5′-UTR, and is translated at a lower level compared to the downstream genes. This regulatory unit may be quite widespread in bacteria, since several other taxonomically diverse lineages have adjacent acuR-like and acuI-like genes; these operons also have no 5′ leader sequences or ribosomal binding sites and their predicted cis-acting regulatory sequences resemble those of R. sphaeroides acuR-acuI-dddL.
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影响因子：
2
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作者：
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作者：
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