Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg2+-contaminated environments
Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg2+-contaminated environments
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DOI：
10.1128/aem.63.6.2442-2445.1997
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发表时间：
1997-06-01
影响因子：
4.4
通讯作者：
Wilson, DB
中科院分区：
文献类型：
--
作者：
Chen, SL;Wilson, DB
对大肠杆菌菌株进行基因工程改造，使其表达Hg2+转运系统和金属硫蛋白。过量表达酿酒酵母谷胱甘肽s -转移酶融合蛋白或豌豆金属硫蛋白可显著增加MerT和MerP运输的Hg2+的生物积累，保护细胞免受积累的Hg2+的影响。重组菌株对Hg2+污染环境具有良好的生物修复性能。
Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein significantly increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent properties for bioremediation of Hg2+-contaminated environments.