Effects of Exogenous Synthetic Autoinducer-2 on Physiological Behaviors and Proteome of Lactic Acid Bacteria
Effects of Exogenous Synthetic Autoinducer-2 on Physiological Behaviors and Proteome of Lactic Acid Bacteria
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DOI：
10.1021/acsomega.9b01021
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发表时间：
2020-01-28
期刊：
影响因子：
4.1
通讯作者：
He, Yinfeng
中科院分区：
文献类型：
--
作者：
Gu, Yue;Wu, Jing;He, Yinfeng
细菌群体使用细胞间通讯系统来协调整个群落的调节过程，这被称为群体感应（QS）。自诱导物-2（Autoinducer-2，AI-2）是一种普遍存在的信号分子，在不同的细菌中介导种间和种内QS系统。研究了外源添加人工合成的AI-2对乳酸菌生理行为和蛋白质组的影响。外源AI-2对屎肠球菌8-3细胞密度有浓度依赖性影响。在E.屎菌8-3和发酵乳杆菌2-1。但提高了E.屎囊8-3。加入AI-2后，在大肠杆菌中鉴定出15个差异表达蛋白。faecium 8-3，它们参与RNA转运信号传导、RNA聚合酶、核糖体、氧化磷酸化、半胱氨酸和甲硫氨酸代谢、嘧啶代谢、ATP结合盒（ABC）转运蛋白、嘌呤代谢、氨基酸生物合成途径等。其中，5-甲硫腺苷/S-腺苷高半胱氨酸核苷酶的表达，其已知参与AI-2合成以及半胱氨酸和氨基酸代谢。这些发现将为阐明AI-2介导的细胞间通讯机制和细菌生理行为奠定基础。
Bacterial populations use a cell-to-cell communication system to coordinate community-wide regulation processes, which is termed quorum sensing (QS). Autoinducer-2 (AI-2) is a universal signal molecule that mediates inter- and intraspecies QS systems among different bacteria. In this study, the effects of exogenous addition of AI-2 synthesized in vitro on physiological behaviors and proteome were investigated in lactic acid bacteria strains. Exogenous AI-2 had a concentration-dependent effect on the Enterococcus faecium 8-3 cell density. There was no significant influence on biofilm formation and individual morphology of ce I k upon 60 mu M AI-2 addition in E. faecium 8-3 and Lactobacillus fermentum 2-1. However, it improved the acid and alkali resistance of E. faecium 8-3. With the addition of AI-2, 15 differentially expressed proteins were identified in E. faecium 8-3, which participate in RNA transport signaling, RNA polymerase, ribosome, oxidative phosphorylation, cysteine and methionine metabolism, pyrimidine metabolism, ATP-binding cassette (ABC) transporters, purine metabolism, biosynthesis of the amino acid pathway, etc. Among them, the expression of 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase, which is known to be involved in AI-2 synthesis and cysteine and amino acid metabolism, was upregulated. These findings will lay the foundation to clarify the mechanism of cell-to-cell communication and bacterial physiological behaviors mediated by AI-2.