Cryptosporidium species:: Preliminary descriptions of the prevalence and genotype distribution among school children and hospital patients in the Venda region, Limpopo Province, South Africa
Cryptosporidium species:: Preliminary descriptions of the prevalence and genotype distribution among school children and hospital patients in the Venda region, Limpopo Province, South Africa
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DOI：
10.1016/j.exppara.2006.04.007
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发表时间：
2006-12-01
影响因子：
2.1
通讯作者：
Guerrant, R. L.
中科院分区：
文献类型：
--
作者：
Samie, A.;Bessong, P. O.;Guerrant, R. L.
在本研究中，隐孢子虫的患病率和物种分布在南非文达地区的学龄儿童和医院病人。真实的时间PCR（qPCR）用于初始筛选，以检测阳性样品，而巢式PCR随后限制性片段长度多态性用于确定物种基因型。在总共检测的244份粪便样本中，44份（18%）有隐孢子虫，其中36/197份（18%）来自医院的患者样本和8/47份（17%）来自小学的样本之间没有显著差异（χ（2）= 0.04; P = 0.841）。受影响最大的年龄组是2至5岁（28.6%）和50至59岁（50.0%）。在31例HIV阳性者中，4例（12.5%）检出隐孢子虫。57%的隐孢子虫阳性样品是嗜热的，26个（59.1%）具有升高的乳铁蛋白含量。C.人型（82%）较C.小孢子虫（18%）。这项研究证明了文达地区隐孢子虫感染的高患病率及其引起腹泻和炎症的影响。(c)2006年爱思唯尔公司All rights reserved.
In the present study, the prevalence and species distribution of Cryptosporidium among school children and hospital patients in the Venda region of South Africa was determined. Real time PCR (qPCR) was used for initial screening to detect positive samples while a nested PCR followed by restriction fragment length polymorphism was used to determine the species genotype. From a total of 244 stool samples tested, 44 (18%) had Cryptosporidium with no significant difference (chi(2) = 0.04; P = 0.841) between samples collected from patients attending hospitals 36/197 (18%) and the samples from primary schools 8/47 (17%). The age groups most affected were those from 2 to 5 years old (28.6%) and 50 to 59 years old (50.0%). Cryptosporidium was detected in 4 (12.5%) of the 31 HIV positive individuals. Fifty-seven percent of the Cryptosporidium positive samples were diarrheic and 26 (59.1%) had elevated lactoferrin content. C. hominis (82%) was more common than C. parvum (18%). This study has demonstrated the high prevalence of Cryptosporidium infections in the Venda region and its implications in causing diarrhea and inflammation. (c) 2006 Elsevier Inc. All rights reserved.