Quantitative proton spectroscopy and histology of a canine brain tumor model.
Quantitative proton spectroscopy and histology of a canine brain tumor model.
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犬脑肿瘤模型的定量质子光谱和组织学。
DOI：
10.1002/mrm.1910300408
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发表时间：
1993
影响因子：
3.3
通讯作者：
Anderson,JH
中科院分区：
文献类型：
--
作者：
Barker,PB;Blackband,SJ;Chatham,JC;Soher,BJ;Samphilipo,MA;Magee,CA;Hilton,JD;Strandberg,JD;Anderson,JH
对8只植入Wodinsky （Proc. Am.）可移植犬胶质瘤模型的狗进行定量、单体素质子核磁共振（NMR）和组织学分析。Assoc。癌症杂志，1999,99（1969）。来自胆碱、肌酸、N -乙酰天冬氨酸（NAA）和乳酸的信号被转换成摩尔浓度单位，并与核磁共振光谱选定的局部体积内组织学确定的组织类型的定量分析相关联。总的来说，与正常大脑相比，病变与所有代谢物浓度的降低有关，乳酸盐除外，它增加了。NAA和肌酸的减少与病变总体积呈极显著相关（P< 0.01），表明这些化合物仅存在于正常脑内。胆碱水平的变化与任何特定的组织类型没有强烈的相关性。乳酸含量随病变总体积的增加而增加（P< 0.01），但不随肿瘤百分比的增加而增加，提示其在肿瘤以外的异常组织中积累。所报道的光谱与在人类胶质母细胞瘤中观察到的相似，除了没有观察到胆碱的升高。可移植犬胶质瘤系统似乎是临床放射学技术（如磁共振成像（MRI）和质子核磁共振波谱）评估的合适肿瘤模型。
Quaintitative, single voxel proton nuclear magnetic resonance (NMR) spectroscopy and histological analysis was performed in eight dogs implanted with the transplantable canine glioma model of Wodinsky (Proc. Am. Assoc. Cancer Res.10, 99 (1969). Signals from choline, creatine,N‐Acetyl Aspartate (NAA) and lactate were converted to molar concentration units and correlated with the quantitative analysis of histologically determined tissue types within the localized volume selected for NMR spectroscopy. In general, compared with normal brain, the lesions were associated with reductions in all metabolite concentrations, with the exception of lactate, which was increased. NAA and creatine decreases were most significantly correlated with the total lesion volume (P< 0.01), suggesting that these compounds are present in normal brain only. Changes in choline levels did not correlate strongly with any particular tissue type. Lactate was found to increase with increasing total lesion volume (P< 0.01), but not with increasing percent tumor, suggesting that it accumulates in abnormal tissue other than the tumor. The spectra reported were similar to those observed in human glioblastomas, with the exception that elevations of choline were not observed. The transplant‐able canine gliosarcoma system appears to be a suitable tumor model for evaluation by clinical radiological techniques such as magnetic resonance imaging (MRI) and proton NMR spectroscopy.