Anaerobic degradation of flavonoids by Eubacterium ramulus
Anaerobic degradation of flavonoids by Eubacterium ramulus
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DOI：
10.1007/s002030050010
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发表时间：
2000-01-01
影响因子：
2.8
通讯作者：
Blaut, M
中科院分区：
文献类型：
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作者：
Schneider, H;Blaut, M
分枝真杆菌（Eubacterium ramulus）是一种降解槲皮素-3-葡萄糖苷的厌氧微生物，在人类的干粪便中大约有10(8)/g的数量，对其转化其他类黄酮的能力进行了测试。该生物将木犀草素-7-葡萄糖苷、芦丁、槲皮素、山奈酚、木犀草素、周周醇、柚皮素、杉木素和根皮素降解为酚酸。它将山奈酚-3-山奈酚-7-葡萄糖苷水解为山奈酚-3-山奈酚-3-山奈酚苷，并将槲皮素厌氧降解产物3,4-二羟基苯乙酸缓慢转化为非芳香发酵产物。木黄素-5-葡萄糖苷、薯蓣皂苷-7-芦丁苷、柚皮素-7-新橙皮苷、(+)-儿茶素和(-)-表儿茶素未被降解。分枝藤细胞提取物具有α -和β - d -葡萄糖苷酶活性，但缺乏α - l -鼠李糖苷酶活性。根据这些底物的降解模式，提出了小枝黄酮的降解途径。
Eubacterium ramulus, a quercetin-3-glucoside-degrading anaerobic microorganism that occurs at numbers of approximately 10(8)/g dry feces in humans, was tested for its ability to transform other flavonoids. The organism degraded luteolin-7-glucoside, rutin, quercetin, kaempferol, luteolin, eriodictyol, naringenin, taxifolin, and phloretin to phenolic acids. It hydrolyzed kaempferol-3-sorphoroside-7-glucoside to kaempferol-3-sorphoroside and transformed 3,4-dihydroxyphenylacetic acid, a product of anaerobic quercetin degradation, very slowly to non-aromatic fermentation products. Luteolin-5-glucoside, diosmetin-7-rutinoside, naringenin-7-neohesperidoside, (+)-catechin, and (-)-epicatechin were not degraded. Cell extracts of E. ramulus contained alpha- and beta-D-glucosidase activities, but were devoid of alpha-L-rhamnosidase activity. Based on the degradation patterns of these substrates, a pathway for the degradation of flavonoids by E. ramulus is proposed.