Thirty-Femtogram Detection of Iron in Mammalian Cells
Thirty-Femtogram Detection of Iron in Mammalian Cells
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DOI：
10.1002/smll.201102356
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发表时间：
2012-07-09
期刊：
影响因子：
13.3
通讯作者：
Berret, Jean-Francois
中科院分区：
文献类型：
--
作者：
Galimard, Aymeric;Safi, Malak;Berret, Jean-Francois
目前正在开发具有增强的光学、机械或磁性属性的无机纳米材料和颗粒，以用于广泛的应用。然而，关于其潜在的细胞和基因毒性的安全问题已经出现。对于体内和体外实验，最近的发展更加需要简单便捷的方法来测量细胞或组织吸收的纳米粒子的量。在这项工作中，报道了一种快速且高度灵敏的方法来定量哺乳动物细胞中氧化铁纳米颗粒的摄取。该方法利用浓盐酸反应物和基于比色的紫外-可见吸收技术消化孵育的细胞。该技术可以在 4 个十年内检测细胞中的铁，质量范围为每个细胞 0.03 至 300 皮克。该协议应用于不同表面化学和尺寸的颗粒，表明涂层是纳米颗粒/细胞相互作用的关键参数。这些数据通过扫描和透射电子显微镜得到证实，结果强调了质膜上弹性吸附纳米颗粒的重要性。
Inorganic nanomaterials and particles with enhanced optical, mechanical, or magnetic attributes are currently being developed for a wide range of applications. Safety issues have developed however concerning their potential cyto- and genotoxicity. For in vivo and in vitro experimentations, recent developments have heightened the need for simple and facile methods to measure the amount of nanoparticles taken up by cells or tissues. In this work, a rapid and highly sensitive method for quantifying the uptake of iron oxide nanoparticles in mammalian cells is reported. The approach exploits the digestion of incubated cells with concentrated hydrochloric acid reactant and a colorimetric-based UV-visible absorption technique. The technique allows the detection of iron in cells over 4 decades in masses from 0.03 to 300 picograms per cell. Applied on particles of different surface chemistry and sizes, the protocol demonstrates that the coating is the key parameter in the nanoparticle/cell interactions. The data are corroborated by scanning and transmission electron microscopy, and the results stress the importance of resiliently adsorbed nanoparticles at the plasma membrane.