Neutrophil expression of CD11b/CD18 and IL-8 secretion during normothermic cardiopulmonary bypass
Neutrophil expression of CD11b/CD18 and IL-8 secretion during normothermic cardiopulmonary bypass
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DOI：
10.1016/s1053-0770(97)90007-0
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发表时间：
1997-08-01
影响因子：
2.8
通讯作者：
Philip, I
中科院分区：
文献类型：
--
作者：
Paugam, C;CholletMartin, S;Philip, I
目的：为了评估常温体外循环（CPB）过程中血液中性粒细胞（PMN）的活化状态，测定了PMN粘附分子CD 11b/CD 18的表达。研究了中性粒细胞的基础状态以及体外被细菌肽（FMLP）刺激的能力。由于白细胞介素-8（IL-8）已知在体外诱导PMN中的CD 11b/CD 18表达，因此同时测量IL-8血浆水平。设计：前瞻性研究。设置：大学医院。参与者：13名计划进行心脏手术的患者。干预措施：在CPB开始后的前4小时内同时抽取全身动脉和肺动脉血液样本。测量和主要结果：CPB开始后20分钟，PMN CD 11b/CD 18的基础表达上调，而IL-8血浆水平保持不变。PMN CD 11b表达的增加一直持续到CPB开始后4小时。此时，IL-8血浆水平升高最大，即使在主动脉开放后，肺动脉和体动脉IL-8血浆水平之间也没有发现差异。PMNFMLP体外刺激的能力保持normal.Conclusions：常温CPB诱导CD 11b表达的快速增加，这似乎是类似于低温期间观察到的。IL-8可能与肺再灌注早期CD 11b表达上调无关，但可能参与了肺再灌注过程中PMN的隔离，并维持PMN CD 11b的表达。虽然部分激活，但PMNs保持对进一步FMLP刺激的正常反应能力，从而对细菌感染作出反应。版权所有（C）1997 W.B.桑德斯公司
Objective: To assess blood polymorphonuclear neutrophil (PMN) activation status during normothermic cardiopulmonary bypass (CPB), the expression of the PMN adhesion molecule CD11b/CD18 was measured. Basal state as well as ex vivo capacity of PMN to be stimulated by a bacterial peptide (FMLP) were investigated. Because interleukin-8 (IL-8) is known to induce CD11b/CD18 expression in vitro in PMN, IL-8 plasma levels were concomitantly measured.Design: Prospective study.Setting: University hospital.Participants: Thirteen patients scheduled for cardiac surgery.Interventions: Systemic arterial and pulmonary arterial blood samples were withdrawn at the same moment during the first 4 hours after the onset of CPB.Measurements and Main Results: Twenty minutes after the onset of CPB, basal expression of PMN CD11b/CD18 was upregulated, whereas IL-8 plasma levels remained unchanged. The increase in PMN CD11b expression was maintained until the fourth hour after the onset of CPB. At this time, elevation of IL-8 plasma levels was maximal, No differences were found between pulmonary and systemic arterial IL-8 plasma levels, even after aortic unclamping. The capacity of PMN to be stimulated ex vivo by FMLP remained normal.Conclusions: Normothermic CPB induced a fast increase in CD11b expression, which appeared to be similar to that observed during hypothermia. IL-8 was probably not related to the very early CD11b upregulation, but could be involved in pulmonary PMN sequestration during pulmonary reperfusion and contribute to the maintained expression of PMN CD11b. Although partially activated, PMNs maintain a normal capacity to respond to a further FMLP stimulation and thus to bacterial infection. Copyright (C) 1997 by W.B. Saunders Company.