Mussel-inspired hybrid coating functionalized porous hydroxyapatite scaffolds for bone tissue regeneration
Mussel-inspired hybrid coating functionalized porous hydroxyapatite scaffolds for bone tissue regeneration
复制标题
受贻贝启发的混合涂层功能化多孔羟基磷灰石支架用于骨组织再生
DOI：
10.1016/j.colsurfb.2019.04.024
复制
发表时间：
2019-07-01
影响因子：
5.8
通讯作者：
Lu, Xiong
中科院分区：
文献类型：
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作者：
Han, Lu;Jiang, Yanan;Lu, Xiong
骨组织工程支架材料应具有良好的孔隙率、力学性能、细胞亲和性和与生物活性物质结合诱导细胞分化的能力。在这项研究中，我们成功地制备了多孔羟基磷灰石（HA）支架功能的聚（L-赖氨酸）/聚多巴胺（PLL/PDA）杂化涂层。PLL/PDA涂层利用了PDA的高蛋白和细胞亲和性以及PLL的生物降解性。因此，该涂层可以在温和条件下通过邻苯二酚化学将骨形态发生蛋白2（BMP 2）锚定到HA支架上，从而保护BMP 2的生物活性。同时，当PLL在生理环境中降解时，涂层还可以以可调和可持续的方式释放BMP 2。在HA支架上的BMP 2包埋的PLL/PDA涂层可以更有效地促进骨髓基质细胞（BMSC）在体外的成骨分化，并诱导异位骨形成到一个更大的水平在体内相比，裸HA支架，提供BMP 2在一个爆裂的方式。所有这些结果表明，PDA介导的儿茶酚修饰的HA支架可以是一个有效的策略，开发可持续的蛋白质递送系统，PLL/PDA涂层的HA支架可能是一个有前途的候选人，骨组织工程应用。
The scaffold for bone tissue engineering should possess proper porosity, adequate mechanical properties, cell affinity for cell attachment, and the capability to bind bioactive agents to induce cell differentiation. In this study, we successfully prepared a porous hydroxyapatite (HA) scaffold that is functionalized by poly(L-lysine)/polydopamine (PLL/PDA) hybrid coating. The PLL/PDA coating takes advantages of the high protein and cell affinity of PDA, as well as the biodegradability of PLL. Therefore, the coating can anchor bone morphogenic protein-2 (BMP2) to the HA scaffold via catechol chemistry under a mild condition so as to protect the bioactivity of BMP2. Meanwhile, the coating can also release BMP2 in a tunable and sustainable manner as the PLL degrades in the physiological environment. The BMP2-entrapped PLL/PDA coating on the HA scaffold can more efficiently promote osteogenic differentiation of bone marrow stromal cells (BMSCs) in vitro and induce ectopic bone formation to a much greater level in vivo compared with a bare HA scaffold that delivers BMP2 in a burst manner. All of these results suggest that the PDA-mediated catechol modification of the HA scaffold can be an effective strategy to develop sustainable protein delivery system, and that the PLL/PDA-coated HA scaffold could be a promising candidate for bone tissue engineering applications.