Relaxation-Based Magic-Angle Spinning NMR Approaches for Studying Protein Dynamics
Relaxation-Based Magic-Angle Spinning NMR Approaches for Studying Protein Dynamics
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DOI：
10.1002/9780470034590.emrstm1417
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Lewandowski, Jozef R.
中科院分区：
文献类型：
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作者：
Lamley, Jonathan M.;Lewandowski, Jozef R.
固态魔角自旋（MAS）NMR弛豫测量提供了一个强大的工具，表征蛋白质动力学。我们通过回顾基本理论概念、实验考虑和定量分析的示例程序，对当代基于弛豫的MAS NMR方法进行了一般性介绍。我们讨论的方法适用于表征发生在时间尺度上的运动范围从皮秒到毫秒。我们也勾勒出策略，以获得时间尺度，幅度和方向的分子运动。
Solid-state magic angle spinning (MAS) NMR relaxation measurements provide a powerful tool for characterizing protein dynamics. We provide a general introduction to contemporary relaxation-based MAS NMR approaches by reviewing the basic theoretical concepts, experimental considerations, and example procedures for quantitative analysis. We discuss methods suitable for characterization of motions occurring on time scales in the range from picoseconds to milliseconds. We also sketch out strategies to obtain time scales, amplitudes, and directions of molecular motions.