Acute toxicity tests using rotifers. III. Effects of temperature, strain, and exposure time on the sensitivity ofBrachionus plicatilis
Acute toxicity tests using rotifers. III. Effects of temperature, strain, and exposure time on the sensitivity ofBrachionus plicatilis
复制标题
使用轮虫进行急性毒性试验。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
G. Persoone
中科院分区：
文献类型：
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作者：
T. Snell;B. Moffat;C. Janssen;G. Persoone
作为标准化急性毒性试验的一部分，研究了包囊年龄、菌株、温度和暴露时间对21种化学物质对河口褶皱臂尾轮虫毒性的影响。毒性具有化学特异性，对小白鼠的半数致死浓度为0.061 mg·L−1，对2，4-二氯苯氧基乙酸的半数致死浓度为598 mg·L−1。实验室内变异系数平均为11%，至少比其他水生无脊椎动物急性试验低三倍。保存长达27个月的轮虫包囊对实验动物的敏感性没有影响，但不同轮虫品系的敏感性存在显著差异。当毒物暴露时间从24小时增加到72小时时，镉和五氯苯酚的LC_(50)值下降了80-90%。对对照组轮虫存活的生命表分析表明，72小时是最长的急性试验。轮虫试验与海胆早期胚胎、海胆精子细胞、Microtox®和Mysidopsis Bahia试验的敏感性比较显示，几种化合物具有可比性。然而，没有任何物种对所有化合物都是最敏感的。
As part of the development of a standardized acute toxicity test, the effect of cyst age, strain, temperature, and exposure time on the toxicity of 21 chemicals to the estuarine rotifer Brachionus plicatilis was investigated. Toxicity was chemical specific, with LC50s ranging from 0.061 mg · L−1 for mecury to 598 mg · L−1 for 2,4-dichlorophenoxyacetic acid. Intralaboratory coefficients of variation averaged 11%, at least three times lower than for other aquatic invertebrate acute tests. The age of rotifer cysts stored up to 27 months had no effect on the sensitivity of test animals, but significant differences in sensitivity were detected among rotifer strains. Test temperatures of 25, 30, and 35°C generally yielded lower LC50s than at 20°C. LC50s decreased by 80–90% for cadmium and pentachlorophenol when toxicant exposure time was increased from 24 to 72 h. Life table analysis of rotifer survival in the controls revealed that 72 h is the longest acute test possible without feeding. A comparison of the sensitivity of the rotifer test to that of sea urchin (Arabacia punctulata) early embryo, sea urchin sperm cell, Microtox®, and Mysidopsis bahia tests revealed comparability for several compounds. However, no species is consistently the most sensitive to all compounds.