Detection of Bacterial Quorum Sensing Molecules.

Detection of Bacterial Quorum Sensing Molecules.
复制标题

细菌群体感应分子的检测

DOI:
10.1007/978-1-4939-7604-1_14
复制
发表时间:
2018
影响因子:
--
通讯作者:
Schikora A
Schikora A
中科院分区:
--
文献类型:
--
作者:
Stein E;Schikora A

文献摘要

相似文献

细菌细胞使用群体感应系统来相互交流。革兰氏阴性菌经常使用N-酰基高丝氨酸内酯用于此目的。检测这些分子的最简单的方法之一是使用特定的报告菌株,其在AHL响应启动子的控制下具有不同种类的报告基因。在这里,我们提出了一些今天可用的可能性,即使不是专门的研究人员。
Bacterial cells use the quorum sensing system to communicate with each other. The gram-negative species very often useN-acyl homoserine lactones for this purpose. One of the easiest ways to detect these molecules is the use of particular reporter strains, which possess different kinds of reporter genes under the control of AHL-responsive promoters. Here we present some of the possibilities available today, even for not specialized researchers.