Non-destructive assessment of inhibition of demineralization in dental enamel irradiated by a λ=9.3-μm CO2 laser at ablative irradiation intensities with PS-OCT
Non-destructive assessment of inhibition of demineralization in dental enamel irradiated by a λ=9.3-μm CO2 laser at ablative irradiation intensities with PS-OCT
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DOI：
10.1002/lsm.20633
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发表时间：
2008-07-01
影响因子：
2.4
通讯作者：
Fried, Daniel
中科院分区：
文献类型：
--
作者：
Can, Anna M.;Darling, Cynthia L.;Fried, Daniel
背景和目的：偏振敏感光学相干断层扫描（PS-OCT）具有很大的希望，用于非破坏性评估防龋剂，如氟化物和热激光治疗对牙釉质表面的疗效。本研究的目的是证明，PS-OCT可用于测量脱矿的陨石坑/切口准备在釉质中的CO2激光操作所需的腔preparations.Materials和方法：在牛牙釉质表面的切口由CO2激光用水喷雾。激光器工作在λ = 9.3 μ m，脉冲持续时间为15 ps，入射能量密度为20 J/cm（2）。激光治疗还与局部氟化物治疗相结合。使用在1，310 nm下操作的PS-OCT系统来获取六个区域的偏振分辨图像，所述六个区域包括每个样品上的声音和激光烧蚀+局部氟化物处理区。在对牙齿成像后，将它们切片，并用偏振光显微镜（PLM）和横向显微放射照相术（TMR）检查薄切片。对15个样本的每一个区域获取PS-OCT扫描的积分反射率和损伤深度、TMR测量的积分矿物质损失和深度以及PLM测量的损伤深度以进行比较。
Background and Objective: Polarization sensitive optical coherence tomography (PS-OCT) has great promise for the non-destructive assessment of the efficacy of anti-caries agents such as fluoride and thermal laser treatments on enamel surfaces. The purpose of this study was to demonstrate that PS-OCT can be used to measure demineralization in craters/incisions prepared in enamel by a CO2 laser operating at the high irradiation intensities required for cavity preparations.Materials and Methods: Incisions in bovine enamel surfaces were produced by a CO2 laser used with a water spray. The laser was operated at lambda = 9.3 mu m with a pulse duration of 15 ps and an incident fluence of 20 J/cm(2). The laser treatments were also combined with topical fluoride treatments. A PS-OCT system operating at 1,310 nm was used to acquire polarization resolved images of six areas including sound and laser-ablated+topical fluoride treated zones on each sample. After imaging the teeth, they were sectioned and the thin sections were examined with polarized light microscopy (PLM) and transverse microradiography (TMR). The integrated reflectivity and lesion depth derived from the PS-OCT scans, the integrated mineral loss and depth measured using TMR and the lesion depth measured with PLM were acquired for each area on the fifteen samples for comparison.Results: The integrated reflectivity and depth in the areas treated by the laser and fluoride were significantly lower (P