Refreshing DNA-Based Nanoprobes by Alcohol for Heavy Metal Detection: Toward Sustainable Sensing Nanomaterials
Refreshing DNA-Based Nanoprobes by Alcohol for Heavy Metal Detection: Toward Sustainable Sensing Nanomaterials
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DOI：
10.1021/acssuschemeng.2c05833
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发表时间：
2023-01
期刊：
影响因子：
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通讯作者：
Luyang Wang;Zhiyu He;Qianyuan Chen;Guoqing Wang;Xingguo Liang;T. Takarada;M. Maeda
中科院分区：
文献类型：
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作者：
Luyang Wang;Zhiyu He;Qianyuan Chen;Guoqing Wang;Xingguo Liang;T. Takarada;M. Maeda
一次性纳米材料的应用不断增加，但潜在的环境风险及其产生的纳米废物已成为全球公众关注的问题。然而，从纳米废物中有效回收有用材料目前受到其微小尺寸、高物理化学活性和不可预测的聚集的限制。我们开发了一种概念性新策略，通过酒精溶剂“刷新”来回收纳米探针。具体来说，DNA功能化的金纳米粒子和纳米棒分别用作Hg(II)和Ag(I)离子的比色纳米探针，这两者都依赖于靶标诱导的DNA末端碱基配对和随后的纳米探针的碱基对堆积组装。然而，当向“废物”聚集体中引入异丙醇时，DNA 会发生变性，释放出重金属离子，从而导致单个纳米探针的恢复。 DNA 杂交的精细调节不仅提供了灵敏的检测，而且还解决了纳米探针高效率（>80%）的回收问题，同时保持检测性能，无论纳米探针的形状和表面移植的 DNA 序列如何。我们进一步展示了回收的纳米探针在鲑鱼肌肉组织中 Hg(II) 物种检测中的用途。凭借回收方法的通用性和可再生纳米探针的综合特征，包括高回收效率和保留的功能，可持续传感纳米材料是指日可待的。
Increasing the applications of disposable nanomaterials yet with potential environmental risk and the resulting nanowastes have become a problem of public concern worldwide. However, efficient recovery of useful materials from nanowastes is currently limited by their tiny size, high physicochemical activity, and unpredictable aggregation. We have developed a conceptually new strategy for recycling nanoprobes by alcoholic solvent “refreshing”. In specific, DNA-functionalized gold nanoparticles and nanorods are employed as colorimetric nanoprobes for Hg(II) and Ag(I) ions, respectively, both of which rely upon target-induced DNA terminal base pairing and the subsequent base pair stacking assembly of the nanoprobes. Upon the introduction of isopropanol to the “waste” aggregates, however, the DNAs are denaturalized to release heavy metal ions, leading to restoration of individual nanoprobes. The fine modulation of DNA hybridization not only affords sensitive detection but also addresses the recycling of the nanoprobes with high efficiency (>80%) while preserving the detection performance irrespective of the nanoprobe shape and surface-grafted DNA sequence. We further demonstrate the use of the recycled nanoprobes in the detection of Hg(II) species from salmon muscle tissue. With the generality of the recovering method and the combined features of the renewable nanoprobes, including high recycling efficiency and retained functionality, sustainable sensing nanomaterials are within reach.