Brugia pahangi:: In vivo tissue migration of early L3 alters gene expression
Brugia pahangi:: In vivo tissue migration of early L3 alters gene expression
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DOI：
10.1016/j.exppara.2007.06.007
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发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Klei, Thomas R.
中科院分区：
文献类型：
--
作者：
Chirgwin, Sharon R.;Coleman, Sharon U.;Klei, Thomas R.
在早期丝状线虫迁移过程中发生的事件是寄生虫建立的核心，但很少研究。在沙鼠后肢皮下注射布氏线虫幼虫，可在感染后3 d从腘窝淋巴结（POP）中恢复。它们被称为迁徙幼虫（IDL3）。另外，L3在第3 DPI时从腹腔恢复（IPL3）不迁移。利用IDL3和IPL3减去cDNA文库，发现IDL3和IPL3之间存在不同的基因谱。肌钙蛋白c在IDL3中显著上调，而组织蛋白酶L在IPL3中显著升高。从蚊子（VL3）分离的IDL3、IPL3和L3之间也观察到这些基因和其他基因的mRNA水平差异。这些数据表明，迁徙活动、暴露于潜在不同的寄主环境和/或寄主位置可能是影响幼虫基因表达的重要外部因素。(c) 2007爱思唯尔公司版权所有。
Events occurring during early filarial nematode migrations are central to parasite establishment but rarely studied. Brugia pahangi larvae injected intradermal (ID) into the hind limb of the gerbil (Meriones unguiculatus) can be recovered from the popliteal lymph node (POP) at 3 days post-infection (DPI). They have been designated migrating larvae (IDL3). Alternatively, L3 recovered at 3 DPI from the peritoneal cavity (IPL3) do not migrate. Subtracted cDNA libraries using IDL3 and IPL3 revealed distinct gene profiles between IDL3 and IPL3. Troponin-c was significantly upregulated in IDL3, while Cathepsin L was significantly increased in IPL3. Differences in mRNA levels were also observed with these and other genes between IDL3, IPL3 and L3 isolated from mosquitoes (VL3). These data suggest that migratory activity, exposure to potentially different host environments and/or host location may be important external factors in influencing larval gene expression. (c) 2007 Elsevier Inc. All rights reserved.