Differential alternative splicing expressions of telomerase reverse transcriptase in gastrointestinal cell lines.
Differential alternative splicing expressions of telomerase reverse transcriptase in gastrointestinal cell lines.
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DOI：
10.3892/or.11.1.127
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发表时间：
2004
期刊：
影响因子：
4.2
通讯作者：
K. Nagao;K. Katsumata;Y. Aizawa;N. Saito;Hiroyuki Hirata;Hiroshi Sasaki;Shigeki Yamamoto;K. Hikiji;T. Koiwa;H. Hisatomi
中科院分区：
文献类型：
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作者：
K. Nagao;K. Katsumata;Y. Aizawa;N. Saito;Hiroyuki Hirata;Hiroshi Sasaki;Shigeki Yamamoto;K. Hikiji;T. Koiwa;H. Hisatomi
端粒酶是一种依赖RNA的DNA聚合酶，在真核生物染色体末端维持端粒TTAGGG重复序列的串联排列。其中一种人类端粒酶成分是hTERT，其具有三个可变剪接位点，引入八种hTERTmRNA亚型。这些亚型在胃肠道细胞系中的表达是未知的。我们开发了一种基于PCR的检测这些剪接变异体的方法。在胃癌和肝细胞癌细胞系中，经常检测到γ缺失变体及其组合变体，α和γ，β和γ，以及α，β和γ缺失变体，而在结直肠癌细胞系中未检测到它们。我们的研究结果提供了重要的信息，用于更详细的研究端粒酶活性的调控。
Telomerase is a cellular RNA-dependent DNA polymerase that serves to maintain the tandem arrays of telomeric TTAGGG repeats at eukaryotic chromosome ends. One of the human telomerase components is hTERT, which has three alternative spliced sites that introduce eight isoforms of hTERT mRNA. The expression of these isoforms in gastrointestinal cell lines is unknown. We developed a PCR-based assay for detecting these splicing variants. In gastric and hepatocellular carcinoma cell lines, the gamma deletion variant and its combination variants, alpha- and gamma-, beta- and gamma-, and alpha-, beta- and gamma-deletion variants were frequently detected, while they were not detected in colorectal carcinoma cell lines. Our results provide important information of use for more detailed studies on the regulation of telomerase activity.