Early detection of myocardial reperfusion by assay of plasma MM-creatine kinase isoforms in dogs.
Early detection of myocardial reperfusion by assay of plasma MM-creatine kinase isoforms in dogs.
复制标题
通过测定犬血浆 MM-肌酸激酶亚型来早期检测心肌再灌注。
DOI：
10.1161/01.cir.74.3.567
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发表时间：
1986
期刊：
影响因子：
37.8
通讯作者：
Abendschein,DR
中科院分区：
文献类型：
--
作者：
Devries,SR;Sobel,BE;Abendschein,DR
为了确定是否可以通过血浆中 MM-肌酸激酶 (CK) 亚型的变化及时检测心肌再灌注，在 30 只清醒的狗中诱导冠状动脉闭塞，并在 21 只狗中 1、2、3 或 4 小时后开始再灌注。通过色谱聚焦对连续血浆样品中的 MM-CK、MMA 心肌亚型进行定量。在冠状动脉闭塞之前，MMA 占血浆中总 CK 活性的 13 +/- 7% (SD)。 MMA 百分比 (MMA%) 在再灌注前升高，但在再灌注开始后 30 分钟至 1 小时内显着且一致地增加至峰值 52 +/- 13% (n = 21)。再灌注 1 小时 (1.44 +/- 0.42% min-1)、2 小时 (1.28 +/- 0.45% min-1) 或 3 小时 (1.02 +/- 0.27% min-1)（p 小于 0.001）时，MMA% 的增加速度显着加快，但再灌注 4 小时时则不然 (0.48 +/- 0.34%) min-1) 与未再灌注对照的比率相比 狗（0.29 +/- 0.09% min-1）。此外，MMA% 的增加率既不受总 CK 活性峰值 (r = -.1) 的影响，也不依赖于冠状动脉闭塞后 24 小时组织化学测量的梗塞面积 (r = -.003)。与对照相比，1至3小时再灌注使从冠状动脉闭塞到MMA%峰值的时间缩短，但该指标的确定速度不如MMA%的增加速度。因此，当心肌梗塞发作后早期发生再灌注时，血浆中MMA％增加率的表征允许对心肌再灌注进行迅速、可靠和无创的检测。
To determine whether myocardial reperfusion can be detected promptly by changes in profiles of isoforms of MM-creatine kinase (CK) in plasma, coronary occlusion was induced in 30 conscious dogs and reperfusion was initiated after 1, 2, 3, or 4 hr in 21. The myocardial isoform of MM-CK, MMA, was quantified in serial plasma samples by chromatofocusing. Before coronary occlusion, MMA comprised 13 +/- 7% (SD) of the total CK activity in plasma. The percentage of MMA (MMA%) was elevated before reperfusion, but increased markedly and consistently to a peak of 52 +/- 13% (n = 21) between 30 min and 1 hr after the time of onset of reperfusion. The rate of increase in MMA% was significantly faster with reperfusion at 1 hr (1.44 +/- 0.42% min-1), 2 hr (1.28 +/- 0.45% min-1), or 3 hr (1.02 +/- 0.27% min-1) (p less than .001), but not with reperfusion at 4 hr (0.48 +/- 0.34% min-1) compared with the rate in nonreperfused control dogs (0.29 +/- 0.09% min-1). Furthermore, the rate of increase in MMA% was neither influenced by peak total CK activity (r = -.1) nor dependent on infarct size measured histochemically 24 hr after coronary occlusion (r = -.003). The time from coronary occlusion to the peak of MMA% was reduced by reperfusion at 1 to 3 hr compared with control, but this index was not identified as rapidly as the rate of increase in MMA%. Accordingly, characterization of the rate of increase in MMA% in plasma when reperfusion occurs early after the onset of myocardial infarction permits prompt, reliable, and noninvasive detection of myocardial reperfusion.