Evaluation of the virulence of Aggregatibacter actinomycetemcomitans through the analysis of leukotoxin.
Evaluation of the virulence of Aggregatibacter actinomycetemcomitans through the analysis of leukotoxin.
复制标题
通过白细胞毒素分析评价放线菌聚集杆菌的毒力。
DOI：
10.1007/978-1-0716-0939-2_18
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yasushi Furuichi
中科院分区：
文献类型：
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作者：
Toshiyuki Nagasawa;Satsuki Kato;Yasushi Furuichi
伴放线菌聚集杆菌常见于局限性侵袭性牙周炎和牙周炎伴系统性疾病。放线菌共生菌产生一种白细胞毒素，可诱导人类白细胞凋亡。白细胞毒素表达依赖于编码白细胞毒素的基因的上游序列，可能包括启动子;具有截短/短上游序列的菌株比具有一般/长上游序列的菌株表达更多的白细胞毒素。本章通过PCR分析确定了白细胞毒素启动子的类型，并检测了人单核细胞系THP-1与A.放线菌通过DNA梯状条带的形成、膜的扰动和乳酸脱氢酶的释放等途径与放线菌共生。
Aggregatibacter actinomycetemcomitansis frequently isolated from localized aggressive periodontitis and periodontitis associated with systemic diseases.A. actinomycetemcomitansproduces a leukotoxin, which induces apoptosis in human leukocytes. The leukotoxin expression is dependent on the upstream sequence, likely including the promoter, of the gene encoding leukotoxin; strains with the truncated/short upstream sequence express more leukotoxin than strains with the general/long upstream. This chapter addresses the determination of the type of the leukotoxin promoter by PCR analysis, and detection of the apoptosis in the coculture of human monocyte cell line (THP-1) withA. actinomycetemcomitansby the DNA ladder formation, membrane perturbation, and lactate dehydrogenase release.