Covalent conjugation of proteins onto fluorescent single-walled carbon nanotubes for biological and medical applications.
Covalent conjugation of proteins onto fluorescent single-walled carbon nanotubes for biological and medical applications.
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DOI：
10.1039/d2ma00714b
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发表时间：
2023-02-06
影响因子：
5
通讯作者：
Boghossian, Ardemis A
中科院分区：
文献类型：
--
作者：
Wang, Hanxuan;Boghossian, Ardemis A
单壁碳纳米管（SWCNT）具有有利于生物应用的光学性质，例如传感，递送和成像。这些应用需要大分子的固定，可以作为治疗药物，分子模板，或表面相互作用的调节剂。虽然以前的研究集中在非共价固定策略，最近的进展已经引入共价功能处理，可以保持甚至增强单壁碳纳米管的光学性能。本文综述了单壁碳纳米管的共价侧壁修饰，重点是最新一代的“sp3缺陷”的修改。我们总结和比较这些sp3化学的反应条件和报道的产品。我们进一步审查了潜在的单壁碳纳米管的荧光和应用这些原则，从这些共价修饰的单壁碳纳米管发出的荧光。最后，我们提供了一个展望，可以应用到共价结合蛋白质这些化学修饰，荧光单壁碳纳米管的其他化学。我们回顾了这些方法的优点，进一步改进的新机会，以及它们对实现新技术的影响。最近的进展使共价蛋白质连接到单壁碳纳米管的新化学策略成为可能。
Single-walled carbon nanotubes (SWCNTs) have optical properties that are conducive for biological applications such as sensing, delivery, and imaging. These applications necessitate the immobilization of macromolecules that can serve as therapeutic drugs, molecular templates, or modulators of surface interactions. Although previous studies have focused on non-covalent immobilization strategies, recent advances have introduced covalent functional handles that can preserve or even enhance the SWCNT optical properties. This review presents an overview of covalent sidewall modifications of SWCNTs, with a focus on the latest generation of “sp3 defect” modifications. We summarize and compare the reaction conditions and the reported products of these sp3 chemistries. We further review the underlying photophysics governing SWCNT fluorescence and apply these principles to the fluorescence emitted from these covalently modified SWCNTs. Finally, we provide an outlook on additional chemistries that could be applied to covalently conjugate proteins to these chemically modified, fluorescent SWCNTs. We review the advantages of these approaches, emerging opportunities for further improvement, as well as their implications for enabling new technologies. Recent advances enable new chemical strategies for covalent protein linking to single-walled carbon nanotubes.