The mannose receptor is expressed by subsets of APC in non-lymphoid organs
The mannose receptor is expressed by subsets of APC in non-lymphoid organs
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DOI：
10.1186/1471-2172-6-4
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发表时间：
2005-02-08
期刊：
影响因子：
3
通讯作者：
Linehan, SA
中科院分区：
文献类型：
--
作者：
Linehan, SA
背景资料：甘露糖受体（MR）是M phi和内皮细胞亚群的内吞受体，其天然配体包括自身糖蛋白和微生物聚糖。它也由未成熟培养的树突状细胞（DC）表达，在那里它介导糖基化抗原的高效摄取，但其在体内抗原处理中的作用尚不清楚。了解哪些APC亚群表达MR将有助于实验设计以解决其免疫功能。结果：在小鼠的皮肤、肝脏、心肌、骨骼肌和舌等外周器官中均检测到MR阳性APC。MR阳性细胞在唾液腺，甲状腺和胰腺共表达MHC II类和髓样标志物macrosialin和sialoadhesin，但不是树突状细胞标志物CD 11 c或DEC-205。MR和MHC II类共定位在共聚焦显微镜图像，这意味着抗原捕获可能是MR在这些细胞中的主要作用。结论：MR的组织和亚细胞分布表明MR是APC的高效抗原摄取受体。
Background: The mannose receptor (MR) is an endocytic receptor of M phi and endothelial cell subsets whose natural ligands include both self glycoproteins and microbial glycans. It is also expressed by immature cultured dendritic cells ( DC), where it mediates high efficiency uptake of glycosylated antigens, yet its role in antigen handling in vivo is unknown. Knowledge of which APC subsets express MR will assist the design of experiments to address its immunological functions. Here the expression of MR by MHC class II positive APC in non-lymphoid organs of the mouse is described.Results: MR positive APC were identified in several peripheral organs: skin, liver, cardiac and skeletal muscle and tongue. MR positive cells in salivary gland, thyroid and pancreas coexpressed MHC class II and the myeloid markers macrosialin and sialoadhesin, but not the dendritic cell markers CD11c or DEC-205. MR and MHC class II colocalised in confocal microscope images, implying that antigen capture may be the primary role of MR in these cells. Distinct ligands of MR were found in salivary gland and pancreas tissue lysates that are candidate physiological ligands of MR positive APC in these organs.Conclusions: The tissue and subcellular distribution of MR suggest it is appropriately located to serve as a high efficiency antigen uptake receptor of APC.