ANTIGENIC COMPARISON OF FELINE CORONAVIRUS ISOLATES - EVIDENCE FOR MARKEDLY DIFFERENT PEPLOMER GLYCOPROTEINS
ANTIGENIC COMPARISON OF FELINE CORONAVIRUS ISOLATES - EVIDENCE FOR MARKEDLY DIFFERENT PEPLOMER GLYCOPROTEINS
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DOI：
10.1128/jvi.61.8.2607-2613.1987
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发表时间：
1987-08-01
影响因子：
5.4
通讯作者：
TERAMOTO, YA
中科院分区：
文献类型：
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作者：
FISCUS, SA;TERAMOTO, YA
采用26种单克隆抗体（mab）对7株猫冠状病毒分离株的抗原性关系进行了研究。这些单克隆抗体被分为5个免疫反应组，用于描述两种猫冠状病毒的抗原类型。一种抗原类型包括毒性较强的猫感染性腹膜炎病毒（FIPV）分离物（FIPV- ucd -1、FIPV- ucd -4、FIPV- tn406、FIPV- df2和FIPV-79-1146），而第二种抗原类型由毒性较弱的FIPV- ucd -2分离物组成。猫肠道冠状病毒分离物FECV-79-1683具有这两种主要抗原群的某些特征。核衣壳和包膜多肽上的抗原表位在两种抗原类型中普遍高度保守，尽管一些类型特异性抗原位点被区分开来。最引人注目的发现是在两种抗原类型之间的pplomer （E2）糖蛋白的显著抗原差异。7种抗E2单克隆抗体与一种抗原E2反应，而其他7种抗E2单克隆抗体识别不同的抗原E2。14种抗e2单克隆抗体均不能与所有分离株发生反应。
The antigenic relationships among seven feline coronavirus isolates were investigated by using a panel of 26 monoclonal antibodies (MAbs). The MAbs were categorized into five immunoreactive groups which were used to delineate two antigenic types of feline coronaviruses. One antigenic type included the more virulent feline infectious peritonitis virus (FIPV) isolates (FIPV-UCD-1, FIPV-UCD-4, FIPV-TN406, FIPV-DF2, and FIPV-79-1146), whereas the second antigenic type was composed of the avirulent isolate FIPV-UCD-2. The feline enteric coronavirus isolate FECV-79-1683 shared some characteristics of both of the major antigenic groups. Epitopes on the nucleocapsid and envelope polypeptides were in general highly conserved among both antigenic types, although a few type-specific antigenic sites were discriminated. The most striking finding was the marked antigenic difference in the peplomer (E2) glycoproteins between the two antigenic types. Seven anti-E2 MAbs reacted with one antigenic type of E2, whereas seven other anti-E2 MAbs recognized a different antigenic form of E2. None of the 14 anti-E2 MAbs reacted with all of the isolates.