Production of chlorothalonil hydrolytic dehalogenase fromagro-industrial wastewater and its application in raw food cleaning
Production of chlorothalonil hydrolytic dehalogenase fromagro-industrial wastewater and its application in raw food cleaning
复制标题
利用农工业废水生产百菌清水解脱卤酶及其在生食净化中的应用
DOI：
10.1002/jsfa.8079
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发表时间：
2017-06-01
影响因子：
4.1
通讯作者：
Yan, Xin
中科院分区：
文献类型：
--
作者：
He, Qin;Xu, Xi-hui;Yan, Xin
背景：为降低百菌清水解脱卤酶（Chd）工业化生产的发酵成本，利用包括糖蜜、玉米浆（CSL）和发酵废水在内的农业工业废水替代昂贵的碳源和氮源以及实验室配制所需的淡水。
结果：结果表明，糖蜜和玉米浆分别可替代5%的碳源和100%的有机氮源，并维持相同的发酵水平。利用超滤发酵废水的萃余液进行二次发酵，可达到初始Chd活性的61.03%，而在萃余液和50%原始培养基成分的混合液中培养时，活性可达到96.39%。选取典型的生鲜食品来评估Chd去除百菌清的能力。在室温下经Chd处理2小时后，樱桃番茄和草莓上30mg/kg的百菌清分别去除了97.40%和75.55%，大白菜上50mg/kg的百菌清去除了60.29%。此外，处理后酶的剩余活性保持在78 - 82%，表明其具有重复使用的潜力。
结论：本研究证明了利用农业工业废水大规模生产Chd在成本上的可行性，并展示了Chd在生鲜食品清洗方面的潜力。（C）2016化学工业协会
BACKGROUND: To reduce the fermentation cost for industrialization of chlorothalonil hydrolytic dehalogenase (Chd), agro-industrial wastewaters including molasses, corn steep liquor (CSL) and fermentation wastewater were used to substitute for expensive carbon and nitrogen sources and fresh water for lab preparation.RESULTS: The results showed that molasses and CSL could replace 5% carbon source and 100% organic nitrogen source respectively to maintain the same fermentation level. Re-fermentation from raffinate of ultra-filtered fermentation wastewater could achieve 61.03% of initial Chd activity and reach 96.39% activitywhen cultured in a mixture of raffinate and 50% of original medium constituent. Typical raw foods were chosen to evaluate the chlorothalonil removal ability of Chd. After Chd treatment for 2 h at room temperature, 97.40 and 75.55% of 30 mg kg(-1) chlorothalonil on cherry tomato and strawberry respectively and 60.29% of 50 mg kg(-1) chlorothalonil on Chinese cabbage were removed. Furthermore, the residual activity of the enzyme remained at 78-82% after treatment, suggesting its potential for reuse.CONCLUSION: This study proved the cost-feasibility of large-scale production of Chd from agro-industrial wastewater and demonstrated the potential of Chd in raw food cleaning. (C) 2016 Society of Chemical Industry