Modification of paper properties using carbohydrate-binding module 3 from the Clostridium thermocellum CipA scaffolding protein produced in Pichia pastoris: elucidation of the glycosylation effect
Modification of paper properties using carbohydrate-binding module 3 from the Clostridium thermocellum CipA scaffolding protein produced in Pichia pastoris: elucidation of the glycosylation effect
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DOI：
10.1007/s10570-015-0655-6
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发表时间：
2015-05
期刊：
影响因子：
5.7
通讯作者：
Carla Oliveira;G. Sepúlveda;Tatiana Q. Aguiar;F. M. Gama;L. Domingues
中科院分区：
文献类型：
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作者：
Carla Oliveira;G. Sepúlveda;Tatiana Q. Aguiar;F. M. Gama;L. Domingues
碳水化合物结合模块（CBMs）已成为一种有趣的纤维修饰酶的替代品。由纸浆和纸张制成。CBMs中的糖基化被认为在纤维素纤维的改善中起着关键作用。因此，在这项工作中，研究了非糖基化（CBM3mt）和糖基化（CBM3wt）重组版本的CBM3，这两个版本都是在毕赤酵母中产生的。结合实验表明，CBM3mt对微晶纤维素（Avicel）的亲和力高于CBM3wt。此外，CBM3mt对Whatman纸的疏水性比CBM3wt高得多。然而，两种CBM3s对纸浆和纸张的影响是相同的。CBM3s对桉树的疏水性没有影响。globulusandPinus sylvestrispulps。另一方面，两者都显著提高了e的强度相关性能。Globuluspapersheets，即破裂（高达12%）和拉伸强度（高达10%）指标。这是第一份显示来自mc的CBM3容量的报告。termocellumCipA来修改纸张的性能。结果表明，糖基化不影响cbm3处理纸浆的排水性，也不影响纸浆的性能。因此，糖基化CBM3中的聚糖可能与纤维改善，即更好的髓质排水无关。
The carbohydrate-binding modules (CBMs) have emerged as an interesting alternative to enzymes for fibers modification,e.g.of pulp and paper. Glycosylation in CBMs is thought to have a key role in the improvement of cellulose fibers. Thus, in this work the non-glycosylated (CBM3mt) and glycosylated (CBM3wt) recombinant versions of CBM3 fromClostridium thermocellumCipA—both produced inPichia pastoris—were studied. Binding assays showed that CBM3mt had a higher affinity for microcrystalline cellulose (Avicel) than CBM3wt. In addition, CBM3mt produced a much higher hydrophobization of Whatman paper than CBM3wt. However, the effects of the two CBM3s on pulp and paper were identical. The CBM3s did not affect the drainability ofEucalyptus globulusor a mixture ofE. globulusandPinus sylvestrispulps. On the other hand, both improved significantly strength-related properties ofE. globuluspapersheets, namely burst (up to 12 %) and tensile strength (up to 10 %) indexes. This is the first report showing the capacity of CBM3 fromC. termocellumCipA to modify paper properties. The results showed that glycosylation did not influence the drainage of CBM3-treated pulps nor the properties of the produced papers. Thus, glycans in glycosylated CBM3 may not be related with fiber improvement, namely superior pulp drainage.