Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
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DOI：
10.1186/1472-6793-9-12
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发表时间：
2009-06-24
期刊：
影响因子：
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通讯作者：
Verini-Supplizi, Andrea
中科院分区：
文献类型：
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作者：
Cappelli, Katia;Felicetti, Michela;Verini-Supplizi, Andrea
背景：应激反应是马匹运动员训练中的一个关键因素；它对于动物的性能和保护其免受生理病理性疾病的影响非常重要。在本研究中，使用外周血单核细胞 (PBMC) 研究了对急性剧烈运动反应的分子机制。结果：使用定量实时 PCR (qRT-PCR) 检测基质金属蛋白酶-1 (MMP-1) 和白细胞介素 8 (IL-8) 基因转录水平的修饰，这些基因源自之前的全基因组表达分析。在一项时程实验设计中，在完成 90-120 公里比赛的 10 匹马中发现这两个基因显着上调。结论：这些结果表明 MMP-1 和 IL-8 均参与运动引起的应激反应，这代表了理解对这种现象的适应性反应的起点。
Background: The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders.In this study, the molecular mechanisms involved in the response to acute and strenuous exercise were investigated using peripheral blood mononuclear cells (PBMCs).Results: Quantitative real-time PCR (qRT-PCR) was used to detect modifications in transcription levels of the genes for matrix metalloproteinase-1 (MMP-1) and interleukin 8 (IL-8), which were derived from previous genome-wide expression analysis. Significant up-regulation of these two genes was found in 10 horses that had completed a race of 90-120 km in a time-course experimental design.Conclusion: These results suggest that MMP-1 and IL-8 are both involved in the exercise-induced stress response, and this represents a starting point from which to understand the adaptive responses to this phenomenon.