Establishment of a new diagnostic method for hydropic villi by using TSSC3 antibody.
Establishment of a new diagnostic method for hydropic villi by using TSSC3 antibody.
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TSSC3抗体诊断绒毛积水的新方法的建立
DOI：
10.1111/jog.12100
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kato H
中科院分区：
文献类型：
--
作者：
Wake N;Takao T;Asanoma K;Kato H
根据DNA多态性，共297例绒毛水肿样本被分为雄激素痣、双二倍体或双亲二倍体。本研究纳入了267份适当样本的子集。大多数肉眼诊断的完全性葡萄胎病例在遗传上是雄激素源性的。部分性葡萄胎病例包括30例雄激素性葡萄胎和12例双精性三倍体。对59例肉眼下分类为水流产的病例，遗传学分析显示38例雄激素性痣，7例二亲三倍体和14例双亲二倍体。这些结果表明，需要一种新的诊断方法来管理积水绒毛患者。我们确定了TSSC印记基因，其表达在正常和部分痣绒毛中，但在完整的痣绒毛中沉默。使用TSSC 3抗体的免疫组织化学证明了其作为鉴别诊断方法的有效性。TSSC 3通过TSSC 3/PI 3 K/Akt/Mash 2信号通路在滋养层干细胞向祖细胞和/或迷路滋养层细胞分化中发挥重要作用。
A total of 297 samples of hydropic villi were classified according to DNA polymorphisms as androgenetic moles, dispermic triploids, or biparental diploids. A subset of 267 appropriate samples was included in the study. Most of the macroscopically diagnosed complete mole cases were genetically androgenetic in origin. The partial mole cases consisted of 30 androgenetic moles and 12 dispermic triploids. For the 59 cases macroscopically categorized as hydropic abortion, the genetic analysis revealed 38 androgenetic moles, seven dispermic triploids and 14 biparental diploids. These results showed that a new diagnostic method was required for the management of patients with hydropic villi. We identified the TSSC imprint gene of which expression was shown in normal and partial mole villi but was silenced in complete mole villi. Immunohistochemistry using the TSSC3 antibody demonstrated its efficacy as the differential diagnostic method. TSSC3 play an important role in the differentiation from trophoblast stem cells to progenitors and/or labyrinth trophoblast through the TSSC3/PI3K/Akt/Mash2 signaling pathway.