Patatin-related phospholipase A, pPLAIIIα, modulates the longitudinal growth of vegetative tissues and seeds in rice.
Patatin-related phospholipase A, pPLAIIIα, modulates the longitudinal growth of vegetative tissues and seeds in rice.
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Patatin 相关磷脂酶 A，pPLAIIIα，调节水稻营养组织和种子的纵向生长
DOI：
10.1093/jxb/erv402
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发表时间：
2015-11
影响因子：
6.9
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Liu G;Zhang K;Ai J;Deng X;Hong Y;Wang X
磷脂酶pPLAIIIα在水稻营养生长和生殖生长中起着重要作用，而OspPLAIIIα的组成性高表达抑制纤维素的产生和细胞的伸长。Patatin相关磷脂酶A（pPLA）水解甘油脂以产生脂肪酸和溶血甘油脂。水稻基因组有21个pPLA，分为5个亚科。OspPLAIIIα的过表达导致水稻茎、根、叶、种子、穗和种子长度减少的矮化表型，而OspPLAIIIα敲除植物具有更长的穗和种子。OspPLAIIIα-过表达植物对赤霉素促进的幼苗伸长的敏感性低于野生型和敲除植物。OspPLAIIIα过表达和敲除对赤霉素信号传导过程中生长阻遏物SLENDER 1的表达具有相反的影响。过表达OspPLAIIIα的植物机械强度和纤维素含量降低，但几种纤维素合成酶基因的表达增加。这些结果表明OspPLAIIIα在水稻营养生长和生殖生长中起作用，并且OspPLAIIIα的组成性高活性抑制细胞伸长。在过量表达的植物中赤霉素反应的降低可能是产生各向异性细胞膨胀的纤维素的能力降低的结果。
The phospholipase pPLAIIIα plays an important role in rice vegetative and reproductive growth, and constitutive, high expression of OspPLAIIIα suppresses cellulose production and cell elongation. Patatin-related phospholipase A (pPLA) hydrolyses glycerolipids to produce fatty acids and lysoglycerolipids. The Oryza sativa genome has 21 putative pPLAs that are grouped into five subfamilies. Overexpression of OspPLAIIIα resulted in a dwarf phenotype with decreased length of rice stems, roots, leaves, seeds, panicles, and seeds, whereas OspPLAIIIα-knockout plants had longer panicles and seeds. OspPLAIIIα-overexpressing plants were less sensitive than wild-type and knockout plants to gibberellin-promoted seedling elongation. OspPLAIIIα overexpression and knockout had an opposite effect on the expression of the growth repressor SLENDER1 in the gibberellin signalling process. OspPLAIIIα-overexpressing plants had decreased mechanical strength and cellulose content, but exhibited increases in the expression of several cellulose synthase genes. These results indicate that OspPLAIIIα plays a role in rice vegetative and reproductive growth and that the constitutive, high activity of OspPLAIIIα suppresses cell elongation. The decreased gibberellin response in overexpressing plants is probably a result of the decreased ability to make cellulose for anisotropic cell expansion.