Comparative analysis of the effect of autoclaving and 10% formalin storage on extracted teeth: A microleakage evaluation.
Comparative analysis of the effect of autoclaving and 10% formalin storage on extracted teeth: A microleakage evaluation.
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DOI：
10.4103/0972-0707.53338
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Miglani, Sanjay
中科院分区：
文献类型：
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作者：
Attam, Kanika;Talwar, Sangeeta;Miglani, Sanjay
目的：比较福尔马林和高压灭菌对牙体标本微渗漏的影响。材料和方法：取45颗恒切牙，随机分为3组，每组15颗：对照组拔牙用生理盐水保存，组2用10%福尔马林保存2周，组3用121℃、15psi压力高压灭菌40min。在规定的保存期限后，所有组均在唇面制备V类洞，并用Z100修复剂进行修复。加工和抛光的样品经历了500次热循环。所有标本在亚甲基蓝中浸泡24小时。切片后，使用光学显微镜在10倍的放大倍数下评估修复体边缘的染料渗漏情况。数据采用非参数Kruskal Wallis单因素方差分析。组间比较采用Mann Whitney检验(P<；0.05)。结果：福尔马林组的微渗漏明显低于对照组。高压灭菌组的平均微渗漏量略高，但差异无统计学意义。结论：高压灭菌组和福尔马林储存组对体外微渗漏值均有不同程度的影响。高压灭菌组的结果与对照组的结果更接近，只有微小的差异。
AIM: This study compares the effect of formalin and autoclaving the tooth samples by evaluating microleakage in-vitro.MATERIALS AND METHODS: Forty-five extracted human permanent incisor teeth were taken and randomly divided into three groups (with different methods of storage and disinfection) with 15 teeth each: Group 1: Control-extracted teeth in this group were stored in normal saline, Group 2: the extracted teeth in this group were stored in 10 % formalin for two weeks and Group 3: the extracted teeth were Autoclaved at 121 degrees C, at 15 psi pressure for 40 minutes. In all the groups after the specified storage period, class V cavities were prepared on the labial surface and restoration was performed with Z100 restorative. Finished and polished samples were subjected to 500 cycles of thermocycling. All specimens were immersed in methylene blue for 24 hours. After sectioning, the margins of restoration were evaluated for dye leakage at 10 X magnification, using an optical microscope. Data were subjected to nonparametric Kruskal Wallis one way analysis of variance. Inter-group comparisons were performed using the Mann Whitney test (P < 0.05).RESULTS: The authors found that the microleakage in the formalin group was considerably lower than that in the control group. The autoclave group showed slightly higher mean microleakage, but the difference was not statistically significant.CONCLUSION: Both autoclaving and formalin storage affect, to a varying degree, the microleakage values in vitro. The results in the autoclaving group matched those of the control group more closely, with only a slight difference.