Involvement of intestinal alkaline phosphatase in serum apolipoprotein B-48 level and its association with ABO and secretor blood group types
Involvement of intestinal alkaline phosphatase in serum apolipoprotein B-48 level and its association with ABO and secretor blood group types
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DOI：
10.1016/j.bbrc.2005.12.145
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发表时间：
2006-03-03
影响因子：
3.1
通讯作者：
Komoda, T
中科院分区：
文献类型：
--
作者：
Nakano, T;Shimanuki, T;Komoda, T
肠碱性磷酸酶（IAP）是一种参与乳糜微粒跨细胞转运的蛋白质，其血清水平在ABO血型之间存在差异。在大鼠肠上皮细胞中，IAP与乳糜微粒分泌相关，但大鼠仅表达A型血。目前还不清楚乳糜微粒分泌是否会影响表达多种血型的人。从40名健康受试者的血清样品后获得过夜的快速和3小时后的高脂肪餐，并测定IAP和载脂蛋白B-48（apoB-48），这两种蛋白质专用于肠，虽然只有apoB-48被发现在乳糜微粒。两种蛋白质在没有血液抗原A的受试者中（B和O）比在有这种抗原的受试者中（A和AB）更高;餐前和餐后IAP分别为2.4倍和4.7倍，apo B-48分别为1.5倍和2.0倍。此外，IAP和apoB-48水平在具有分泌型表型的受试者中强烈相关（r > 0.81）。这些结果表明，IAP强烈参与乳糜微粒的形成和脂肪酸代谢可能会改变ABO血型。另外。apoB-48检测中结合ABO血型分型可提高对代谢综合征的诊断价值。(c)2005年爱思唯尔公司All rights reserved.
Serum levels of intestinal alkaline phosphatase (IAP), a protein implicated in transcellular transport of chylomicrons, vary among ABO blood-groups. In rat enterocytes, IAP is associated with chylomicron secretion, but the rat expresses only blood group A. it is not known whether chylomicron secretion may be affected in humans who express Multiple blood group types. Serum samples from 40 healthy subjects were obtained after overnight fast and 3 h after a high-fat meal, and assayed for IAP and apolipoprotein B-48 (apoB-48), both proteins exclusive to intestine, although only apoB-48 is found in chylomicrons. The two proteins were greater in subjects without blood antigen A (B and O) than in those with this antigen (A and AB); 2.4- and 4.7-fold for IAP and 1.5- and 2.0-fold for apoB-48 before and after the meal, respectively. Moreover, IAP and apoB-48 levels were strongly correlated in the subjects with the secretor phenotype (r > 0.81). These results indicate that IAP is strongly involved in chylomicron formation and fatty acid metabolism might change among ABO blood type. In addition. ABO blood type classification in apoB-48 measurement would improve the diagnostic value in the evaluation of metabolic syndrome. (c) 2005 Elsevier Inc. All rights reserved.