Serological evidence for the presence of influenza D virus in small ruminants.
Serological evidence for the presence of influenza D virus in small ruminants.
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DOI：
10.1016/j.vetmic.2015.09.005
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发表时间：
2015-11-18
影响因子：
3.3
通讯作者：
Li F
中科院分区：
文献类型：
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作者：
Quast M;Sreenivasan C;Sexton G;Nedland H;Singrey A;Fawcett L;Miller G;Lauer D;Voss S;Pollock S;Cunha CW;Christopher-Hennings J;Nelson E;Li F
2011年，从有呼吸道疾病症状的病猪中分离出了D型流感病毒（FLUDV），此后这种新病毒也传播给了牛。关于其他农业动物和家禽对FLUDV的易感性知之甚少。这项研究旨在确定其他农场动物，如山羊，绵羊，鸡和火鸡是否可能是这种新出现的流感病毒的宿主。从美国和加拿大不同地理位置的141个小型反刍动物和25个家禽养殖场收集了648份山羊和绵羊血清样品以及250份鸡和火鸡血清样品。使用血凝抑制（HI）试验检查血清样品，并使用血清中和试验进一步分析绵羊和山羊样品。结果表明，13.5%（17/126）的羊场检出了FLUDV抗体，5.2%（29/557）的羊血清样品检出FLUDV抗体。在山羊检测结果中，13.3%（2/15）的被检羊场检出了FLUDV抗体，8.8%（8/91）的被检羊血清样品检出FLUDV抗体。此外，所有检测的家禽血清样本均为FLUDV抗体阴性。我们的数据表明，绵羊和山羊对FLUDV病毒易感，美国多个州已经在这两个物种中感染了这种病毒。这一新发现强调了未来需要在小型反刍动物中监测FLUDV病毒，以更好地了解这种新病毒的起源和天然宿主。
Influenza D virus (FLUDV) was isolated from diseased pigs with respiratory disease symptoms in 2011, and since then the new virus has also been spread to cattle. Little is known about the susceptibility of other agricultural animals and poultry to FLUDV. This study was designed to determine if other farm animals such as goats, sheep, chickens, and turkey are possible hosts to this newly emerging influenza virus. 648 goat and sheep serum samples and 250 chicken and turkey serum samples were collected from 141 small ruminant and 25 poultry farms from different geographical locations in the United States and Canada. Serum samples were examined using the hemagglutination inhibition (HI) assay and the sheep and goat samples were further analyzed using the serum neutralization assay. Results of this study showed FLUDV antibodies were detected in 13.5% (17/126) of the sampled sheep farms, and 5.2% (29/557) of tested sheep serum samples were positive for FLUDV antibodies. For the goat results, the FLUDV antibodies were detected in 13.3% (2/15) of the sampled farms, and 8.8% (8/91) of the tested goat serum samples were positive for FLUDV antibodies. Furthermore, all tested poultry serum samples were negative for FLUDV antibodies. Our data demonstrated that sheep and goat are susceptible to FLUDV virus and multiple states in U.S. have this virus infection already in these two species. This new finding highlights a need for future surveillance of FLUDV virus in small ruminants toward better understanding both the origin and natural reservoir of this new virus.