Crystal structure of elastase–substrate complex at −55 °C
Crystal structure of elastase–substrate complex at −55 °C
复制标题
-55 °C 下弹性蛋白酶-底物复合物的晶体结构
作者：
T. Alber;T. Alber;Gregory A. Petsko;Demetrius Tsernoglou
弹性蛋白酶催化 N-苯甲酯基-L-丙氨酰-对硝基苯酚酯水解过程中特定酰基酶中间体的结构已通过 3.5 Å 分辨率的 X 射线衍射测定。在底物添加和数据收集过程中，通过将晶体冷却至-55°C来稳定酰基酶。
The structure of a specific acyl-enzyme intermediate in the elastase-catalysed hydrolysis of N-carbobenzoxy-L-alanyl-p-nitrophenol ester has been determined by X-ray diffraction at 3.5 Å resolution. The acyl-enzyme was stabilised by cooling the crystal to −55°C during substrate addition and data collection.