A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability
A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability
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DOI：
10.1016/s0039-9140(97)00017-9
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发表时间：
1997-07-01
期刊：
影响因子：
6.1
通讯作者：
Ueno, K
中科院分区：
文献类型：
--
作者：
Ishiyama, M;Miyazono, Y;Ueno, K
合成了一种高水溶性的二磺酸四唑盐4-[3-（2-甲氧基-4-硝基苯基）-2-（4-硝基苯基）-2H-5-四唑]-1，3-苯二磺酸钠。该化合物在1-甲氧基PMS存在下，在中性pH下以良好的产率被NADH还原，以产生在460 nm处吸收的相应甲瓒染料。甲瓒在高于0.1 M的浓度下可溶于水。因此，证明四唑盐可用作NADH的灵敏显色指示剂。它也适用于细胞增殖试验，作为细胞活力指标。(C)1997年Elsevier Science B.V.
A highly water soluble disulfonated tetrazolium salt, 4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt, was synthesized. The compound is reduced by NADH in good yields al neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nm. The formazan is soluble to water at concentrations higher than 0.1 M. The tetrazolium salt thus proved to be useful as a sensitive chromogenic indicator for NADH. It is also applicable to cell proliferation assays as a cell viability indicator. (C) 1997 Elsevier Science B.V.