Demonstration and characterization of an epidermal angiogenic factor.
Demonstration and characterization of an epidermal angiogenic factor.
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DOI：
10.1111/1523-1747.ep12676198
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发表时间：
1973-09
期刊：
影响因子：
--
通讯作者：
J. Wolf;R. Harrison
中科院分区：
文献类型：
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作者：
J. Wolf;R. Harrison
本研究观察了成年仓鼠表皮和表皮匀浆对颊袋微血管的增殖作用。表皮的植入刺激了新血管的快速生长，无论是直接植入还是通过微孔过滤器与血管床分离。真皮、聚乙烯、透析膜、微孔滤膜、表皮置于透析膜上或透析膜内、表皮经热处理灭活后均未见明显血管增生。表皮匀浆，微孔滤液和透析液诱导新血管生长，而真皮的类似制剂则没有。在乙酸乙酯萃取后，发现活性存在于水相，而不存在于有机相，并通过加热或三氯乙酸沉淀去除活性。在4°C下维持7天，血管增殖活性不受影响。这些观察结果被解释为一种特殊的、热不稳定的、可扩散但不可透析的蛋白质“表皮血管生成因子”的证据。
In this study the vasoproliferative effects of adult hamster epidermis and epidermal homogenates on the microvasculature of the hamster cheek pouch were observed. Implants of epidermis stimulated a rapid growth of new vessels both upon direct implantation and when separated from the vascular bed by a microporous filter. No significant vasoproliferation was seen with dermis, polythene, dialysis membrane, Millipore filter, with epidermis placed on or within sheets of dialysis membrane, or when the epidermis had been inactivated by heat treatment. Epidermal homogenates, Millipore filtrates, and dialysis tenates induced new vessel growth whereas similar preparations from dermis did not. Activity was found to be present in the aqueous but not the organic phase following ethyl acetate extraction and was deactivated by heating or removed by precipitation with trichloroacetic acid. Vasoproliferative activity was not affected by maintenance at 4° C for up to 7 days. These observations are interpreted as evidence for a specific, heat-labile, diffusible but nondialyzable protein "epidermal angiogenic factor."