Immunohistochemical detection of gamma-glutamyl transpeptidase in normal human tissue
Immunohistochemical detection of gamma-glutamyl transpeptidase in normal human tissue
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DOI：
10.1177/44.10.8813074
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发表时间：
1996-10-01
影响因子：
3.2
通讯作者：
Frierson, HF
中科院分区：
文献类型：
--
作者：
Hanigan, MH;Frierson, HF
我们开发了一种新的，高度特异性的抗体，定位γ-谷氨酰转肽酶（GGT）在福尔马林固定的部分人体组织。通过对5种不同组织的全细胞匀浆进行免疫染色Western印迹，证明了抗体GGT 129的特异性。通过对正常人体组织中GGT表达的综合研究显示了抗体的效用。这项研究首次揭示了GGT在许多组织中的表达，包括子宫颈内膜、子宫内膜和肾上腺中的腺体。在肾近端小管细胞、肝胆小管和神经系统内的毛细血管内皮细胞表面观察到强免疫反应性。汗腺、前列腺、唾液腺导管、胆管、胰腺腺泡、肠隐窝和睾丸小管中的分泌或吸收细胞也呈GGT阳性。在一些组织中观察到少量染色阳性的基质细胞和GGT阳性组织细胞。对人类胎儿组织的分析表明，GGT在人类和啮齿动物中的发育表达不同。这些发现为进一步研究肿瘤中GGT诱导和GGT表达对肿瘤化疗反应的影响奠定了基础。
We developed a new, highly specific antibody that localizes gamma-glutamyl transpeptidase (GGT) in formalin-fixed sections of human tissue. The specificity of the antibody, GGT129, is demonstrated by Immunostained Western blots of whole cell homogenate from five different tissues. The utility of the antibody is shown by a comprehensive study of GGT expression in normal human tissue. This study reveals GGT expression for the first time in a number of tissues, including glands in the endocervix, endometrium, and adrenals. Strong immunoreactivity was observed on the surface of renal proximal tubule cells, hepatic bile canaliculi, and capillary endothelial cells within the nervous system. Secretory or absorptive cells in sweat glands, prostate, salivary gland ducts, bile ducts, pancreatic acini, intestinal crypts, and testicular tubules were also GGT-positive. Small bands of positively stained stromal cells and GGT-positive histiocytes were seen in some tissues. Analysis of human fetal tissue shows that the developmental expression of GGT differs in humans and rodents. These findings form the basis for further work on GGT induction in tumors and the effect of GGT expression on the response of tumors to chemotherapy.