Structural Characteristics of Short Peptides in Solution
Structural Characteristics of Short Peptides in Solution
复制标题
DOI：
10.2174/092986652012131112121417
复制
发表时间：
2013-12-01
影响因子：
1.6
通讯作者：
Arakawa, Tsutomu
中科院分区：
文献类型：
--
作者：
Ohtake, Satoshi;Kita, Yoshiko;Arakawa, Tsutomu
短肽作为激动或拮抗配体、聚集抑制剂和疫苗以及在许多其它应用中是重要的生物药物。它们在溶液中的行为与球状蛋白质不同。许多短肽是非结构化的，并且倾向于聚集并响应于溶剂环境的变化而经历结构转变，所述溶剂环境包括pH、温度、离子强度、有机溶剂或表面活性剂的存在以及暴露于脂质膜。这种结构转变通常与原纤维或β-淀粉样蛋白形成有关。短肽的这些结构特征对其功能、免疫原性和储存稳定性具有显著影响。
Short peptides are important biopharmaceuticals as agonistic or antagonistic ligands, aggregation inhibitors, and vaccines, as well as in many other applications. They behave differently from globular proteins in solution. Many short peptides are unstructured and tend to aggregate and undergo structural transition in response to changes in solvent environment, including pH, temperature, ionic strength, presence of organic solvents or surfactants, and exposure to lipid membranes. Such structural transitions are often associated with fibril or beta-amyloid formation. These structural characteristics of short peptides have drastic impact on their function, immunogenicity, and storage stability.