FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids.
FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids.
复制标题
基于 FACS 的测序方法使用脑类器官评估细胞类型与基因型关联。
DOI：
10.1007/978-1-0716-3287-1_15
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Lim,ElaineT
中科院分区：
文献类型：
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作者：
Murray,Liam;Olson,MeaganN;Barton,Nathaniel;Dawes,Pepper;Chan,Yingleong;Lim,ElaineT
最近的技术发展已经导致大规模基于转录组学的测序方法的广泛应用，以确定基因型与细胞型的关联。在这里，我们描述了一种基于荧光激活细胞分选（FACS）的测序方法，利用CRISPR/Cas9编辑的马赛克脑类器官来鉴定或验证基因型与细胞类型的关联。我们的方法是高通量和定量的，并使用内部控制来比较不同抗体标记和实验的结果。
Recent technological developments have led to widespread applications of large-scale transcriptomics-based sequencing methods to identify genotype-to-cell type associations. Here we describe a fluorescence-activated cell sorting (FACS)-based sequencing method to utilize CRISPR/Cas9 edited mosaic cerebral organoids to identify or validate genotype-to-cell type associations. Our approach is high-throughput and quantitative and uses internal controls to enable comparisons of the results across different antibody markers and experiments.