Chicken C-type lectin-like receptor B-NK, expressed on NK and T cell subsets, binds to a ligand on activated splenocytes
Chicken C-type lectin-like receptor B-NK, expressed on NK and T cell subsets, binds to a ligand on activated splenocytes
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DOI：
10.1016/j.molimm.2007.08.024
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发表时间：
2008-03-01
影响因子：
3.6
通讯作者：
Goebel, Thomas W.
中科院分区：
文献类型：
--
作者：
Viertlboeck, Birgit C.;Wortmann, Andreas;Goebel, Thomas W.
B-NK是一种具有基于免疫受体酪氨酸的抑制基序的C型凝集素，其位于鸡MHC附近，并且已被描述为潜在的鸡NK细胞受体。我们已经产生了用于免疫和生物化学研究的表位标记的B-NK版本。B-NK表达为高度糖基化的同源二聚体II型跨膜蛋白。利用新开发的B-NK特异性单克隆抗体，分析了B-NK的组织分布。在血液中，B-NK主要存在于γ δ T细胞的小群体上，而在脾脏中，其由α 3 T细胞表达。此外，在经IL-2体外扩增的CD 3（-）CD 8（+）分选的脾细胞和胚胎脾细胞上也发现了B-NK，这两者都类似于鸡NK细胞。为了表征表达B-NK配体的细胞，采用基于BWZ. 36的报告系统，其在配体结合时诱导β-半乳糖苷酶活性。虽然潜在的B-NK配体如MHC I类或C型凝集素B-lec不诱导任何信号，但胰蛋白酶敏感的B-NK配体在佛波醇肉豆蔻酸酯或伴刀豆球蛋白A活化的脾细胞上表达，但未刺激的脾细胞上不表达。总之，B-NK由NK细胞和T细胞亚群表达，并且它结合活化细胞上的配体。(c)2007爱思唯尔有限公司保留所有权利。
B-NK is a C-type lectin with an immunorecptor tyrosine-based inhibition motif, that is located in the vicinity of the chicken MHC and that has been described as a potential chicken NK cell receptor. We have generated an epitope tagged B-NK version for immunization and for biochemical studies. B-NK was expressed as a heavily glycosylated, homodimeric, type II transmembrane protein. With the help of a newly developed B-NK specific mab, the tissue distribution of B-NK has been analyzed. In the blood, B-NK was mainly present on a small population of gamma delta T cells, whereas in spleen it was expressed by a(3 T cells. Moreover, B-NK was also found on CD3(-)CD8(+) sorted splenocytes that were in vitro expanded by IL-2 and on embryonic splenocytes, both of which resemble chicken NK cells. In order to characterize cells expressing B-NK ligands, a BWZ.36 based reporter system was employed, that induced beta-galactosidase activity upon ligand binding. While potential B-NK ligands such as MHC class I or the C-type lectin B-lec did not induce any signal, a trypsin sensitive B-NK ligand was expressed on phorbol myristate or concanavalin A activated splenocytes, but not unstimulated splenocytes. In summary, B-NK is expressed by NK cells and T cell subsets, and it binds to a ligand on activated cells. (c) 2007 Elsevier Ltd. All rights reserved.