NEUTROPHIL KINETICS IN MAN
NEUTROPHIL KINETICS IN MAN
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DOI：
10.1172/jci108517
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发表时间：
1976-01-01
影响因子：
15.9
通讯作者：
FINCH, CA
中科院分区：
文献类型：
--
作者：
DANCEY, JT;DEUBELBEISS, KA;FINCH, CA
开发了一种测量正常人骨髓中中性粒细胞细胞结构的方法，其中中性粒细胞与红细胞的比率由骨髓切片确定，并且通过红细胞铁转换来估计骨髓正常母细胞。由形态学标准定义的中性粒细胞成熟类别得到用 3 H-胸苷标记的骨髓放射自显影照片的支持。通过对每个成熟类别细胞的连续切片进行计数，凭经验得出多重计数误差的校正。 13 名正常人类受试者的正常中性粒细胞与红细胞比率为 1.5±。 0.07。同一受试者中正常成细胞的平均数量估计为 5.07 .+-。 0.84 倍109个细胞/公斤。骨髓中性粒细胞总数（×109细胞/kg）为7.70±。 1.20，有丝分裂后池（后髓细胞、条带和分段形式）为 5.59 .+-。 0.90，有丝分裂池（早幼粒细胞 + 粒细胞）为 2.11.+-。 0.36。骨髓中性粒细胞（总）产量由有丝分裂后骨髓池中的中性粒细胞数量除以其转运时间确定。通过时间源自注射的 3 H-胸苷在循环中性粒细胞中的出现。有丝分裂后池包含5.59.+-。 0.90倍109 中性粒细胞/kg，转运为 6.60 .+-。 0.03天；骨髓中性粒细胞产量计算为0.85倍。每天 109 个细胞/公斤。有效生产量（以 3 H-胸苷标记的循环中性粒细胞的周转率测量）为 0.87.+-。 0.13 倍每天 109 个细胞/公斤。该值与骨髓中性粒细胞产量的计算密切相关。成交量较大，为 1.62.+-。 0.46倍当使用二异丙基氟磷酸-32P标记循环中性粒细胞时，每天获得109个细胞/kg。使用 3H-胸苷和二异丙基氟磷酸盐-32P 双重标记的同源细胞进行的研究表明，32P 标记的回收率较低且 t1/2 [半衰期]较短。
A method was developed for measuring neutrophil cellularity in normal human bone marrow, in which the neutrophil-erythroid ratio was determined from marrow sections and marrow normoblasts were estimated by the erythron Fe turnover. Neutrophil maturational categories, defined by morphologic criteria, were supported by autoradiographs of marrow flashed-labeled with 3H-thymidine. Correction for multiple counting error was empirically derived by counting serial sections through cells of each maturational category. The normal neutrophil-erythroid ratio in 13 normal human subjects was 1.5 .+-. 0.07. The mean number of normoblasts in the same subjects was estimated to be 5.07 .+-. 0.84 .times. 109 cells/kg. Total marrow neutrophils ( .times. 109 cells/kg) were 7.70 .+-. 1.20, the postmitotic pool (metamyelocytes, bands and segmented forms) was 5.59 .+-. 0.90 and the mitotic pool (promyelocytes + myelocytes) was 2.11 .+-. 0.36. Marrow neutrophil (total) production was determined from the number of neutrophils comprising the postmitotic marrow pool divided by their transit time. Transit time was derived from the appearance in circulating neutrophils of injected 3H-thymidine. The postmitotic pool comprised 5.59 .+-. 0.90 .times. 109 neutrophils/kg, and the transit was 6.60 .+-. 0.03 days; marrow neutrophil production was calculated at 0.85 .times. 109 cells/kg per day. Effective production, measured as the turnover of circulating neutrophils labeled with 3H-thymidine, was 0.87 .+-. 0.13 .times. 109 cells/kg per day. This value correlated well with the calculation of marrow neutrophil production. A larger turnover of 1.62 .+-. 0.46 .times. 109 cells/kg per day was obtained when diisopropylfluorophosphate-32P was used to label circulating neutrophils. Studies using isologous cells doubly labeled with 3H-thymidine and diisopropylfluorophosphate-32P demonstrated a lower recovery and shorter t1/2 [half life] of the 32P label.