KINETOCHORE DETECTION IN MICRONUCLEI - AN ALTERNATIVE METHOD FOR MEASURING CHROMOSOME LOSS
KINETOCHORE DETECTION IN MICRONUCLEI - AN ALTERNATIVE METHOD FOR MEASURING CHROMOSOME LOSS
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DOI：
10.1093/mutage/4.2.98
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发表时间：
1989-03-01
期刊：
影响因子：
2.7
通讯作者：
MORLEY, AA
中科院分区：
文献类型：
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作者：
FENECH, M;MORLEY, AA
采用从硬皮病患者血清中获得的抗着丝粒抗体检测细胞分裂阻断的人淋巴细胞微核内的全染色体。用过氧化物酶标记的第二抗体定位一抗，然后用氯化镍修饰二氨基联苯胺反应，得到永久载玻片制备。82% - 92%的秋水仙碱诱导的MN呈着丝酶阳性。年轻人（20-35岁）和老年人（50 - 65岁）自发发生的微核结果表明，42(.+-。6)和50(+-。6)%，分别含有着丝点。也许更新颖的观察是。apprx。12%的x射线诱导的微核是着丝酶阳性，因此可能是整个染色体丢失事件的结果。微核中的着丝点检测提供了一种测量染色体丢失的新方法，因此在鉴定非整倍体诱导剂方面可能很重要。
Whole chromosomes within micronuclei (MN) in cytokinesis-blocked human lymphocytes were detected by using anti-kinetochore antibodies obtained from the seurm of scleroderma patients. The primary antibody was localized using a peroxidase-labelled second antibody followed by a nickel chloride modification of the diamino-benzidine reaction to give a permanent slide preparation. Between 82 and 92% of colchicine-induced MN were shown to be kinetochore-positive. Results for spontaneously occurring micronuclei in young (20-35 years) and elderly (> 65 years) subjects indicated that 42 (.+-. 6) and 50 (.+-. 6)%, respectively, contained kinetochores. Perhaps the more novel observation was that .apprx. 12% of X-ray-induced micronuclei were kinetochore-positive and thus could have been the result of whole chromosome loss events. Kinetochore-detection in micronuclei provides a new approach to measure chromosome loss and therefore may be important in identifying aneuploidy-inducing agents.