Planar fluorescence imaging using normalized data
Planar fluorescence imaging using normalized data
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DOI：
10.1117/1.2136148
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发表时间：
2005-11-01
影响因子：
3.5
通讯作者：
Shih, HA
中科院分区：
文献类型：
--
作者：
Ntziachristos, V;Turner, G;Shih, HA
近年来，由于能够在体内非侵入性地感测分子功能的适当的报告技术的出现，组织的荧光成像获得了显著的关注。迄今为止，两种主要的方法已经用于荧光分子成像，即，落射照明（反射）成像和荧光分子层析成像。透照是过去用于成像组织的替代方法，并且可以类似地有益于荧光分子成像。我们调查的数据归一化方案在反射和transillumination模式和实验表明，归一化transillumination提供了显着的优势，平面反射成像和非归一化的方法。我们的观察，基于幻影和尸检和在体内小鼠测量显示图像质量的改善，上级深度灵敏度，并提高成像精度的非标准化方法检查。归一化平面成像保留了实现的简单性，并可用于改进标准荧光反射成像，并作为更集成和更准确的断层扫描方法的简化替代方案。(C)2005年光学仪器工程师学会。
Fluorescence imaging of tissues has gained significant attention in recent years due to the emergence of appropriate reporter technologies that enable noninvasive sensing of molecular function in vivo. Two major approaches have been used so far for fluorescence molecular imaging, i.e., epi-illumination (reflectance) imaging and fluorescence molecular tomography. Transillumination is an alternative approach that has been employed for imaging tissues in the past and could be similarly beneficial for fluorescence molecular imaging. We investigate data normalization schemes in reflectance and transillumination mode and experimentally demonstrate that normalized transillumination offers significant advantages over planar reflectance imaging and over nonnormalized methods. Our observations, based on phantoms and on postmortem and in vivo mouse measurements display image quality improvement, superior depth sensitivity, and improved imaging accuracy over the nonnormalized methods examined. Normalized planar imaging retains implementation simplicity and could be used to improve on standard fluorescence reflectance imaging and as a simplified alternative to the more integrated and accurate tomographic methods. (C) 2005 Society of Photo-Optical Instrumentation Engineers.