Morphometry of canine cutaneous mast cell tumors
Morphometry of canine cutaneous mast cell tumors
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DOI：
10.1354/vp.40-3-268
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发表时间：
2003-05-01
影响因子：
2.4
通讯作者：
Catao-Dias, TL
中科院分区：
文献类型：
--
作者：
Strefezzi, RD;Xavier, JG;Catao-Dias, TL
24只犬皮肤结节，诊断为肥大细胞瘤细针穿刺活检，并通过苏木精和伊红（HE）和甲苯胺蓝染色的组织病理学分析证实，进行了分析，通过计算机核形态测定的全景和HE染色的细胞病理学载玻片。每个病变检查200个细胞核。研究的形态计量学参数为核面积、平均直径、周长、规则因子和椭圆因子。根据以下形态学特征将病变分级为I（分化良好）、II（中等分化）或III（分化不良）：侵袭性、细胞结构和细胞形态、有丝分裂指数和基质反应。然后将核形态测量结果与组织病理学分级进行比较。核面积、平均直径和周长随组织病理学分级的增加而增加，但统计学分析显示，仅HE染色时II级和III级之间、I级和III级之间有显著性差异（P < 0.01），而全视染色时I级和III级之间有显著性差异（P < 0.05）。椭圆度因子和规则性因子在组织病理学分级之间没有显着差异。结果表明，核形态分析，结合快速，廉价的细胞病理学技术，可以帮助肥大细胞肿瘤分级，从而有助于建立一个更精确的预后和治疗。
Twenty-four canine cutaneous nodules, diagnosed as mast cell tumors by fine-needle aspiration biopsy and confirmed by histopathologic analysis by staining with hematoxylin and eosin (HE) and toluidine blue, were analyzed by computerized nuclear morphometry on panoptic- and HE-stained cytopathology slides. Two hundred nuclei per lesion were examined. The morphometric parameters investigated were nuclear area, mean diameter, perimeter, regularity factor, and ellipticity factor. Lesions were graded as I (well differentiated), II (intermediate differentiation), or III (poorly differentiated) according to the following morphologic features: invasiveness, cellularity and cellular morphology, mitotic index, and stromal reaction. Nuclear morphometric results were then compared with histopathologic grades. Values of nuclear area, mean diameter, and perimeter increased with increase in histopathologic grade, but statistical analysis revealed significant differences only between grades II and III and between grades I and III when HE was used (P < 0.01) and between grades I and III with panoptic stain (P < 0.05). The ellipticity factor and regularity factor did not reveal significant differences between histopathologic grades. The results indicate that nuclear morphometric analysis, in combination with the rapid and inexpensive cytopathology technique, can help in mast cell tumor grading, thus contributing to the establishment of a more precise prognosis and treatment.