Experimental analysis of development, lipid accumulation and gene expression in a high-latitude marine copepod
Experimental analysis of development, lipid accumulation and gene expression in a high-latitude marine copepod
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DOI：
10.1093/plankt/fbad045
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发表时间：
2023-11-11
影响因子：
2.1
通讯作者：
Lenz，Petra H.
中科院分区：
文献类型：
--
作者：
Roncalli，Vittoria;Block，Lauren N.;Lenz，Petra H.
高纬度的桡足动物neocalanus flemingeri利用春季浮游植物的大量繁殖来积累脂肪，以便在食物有限的时期生存下来，并为繁殖提供燃料。在发育过程中的某个时刻，脂质积累结束，前成虫蜕皮为成虫，下降到深处，进入一种称为“滞育”的休眠状态。他们如何以及何时决定进行这种转变仍未得到解决。根据一种假设，触发因素是他们达到了“脂质丰满”的阈值。或者，它们可能遵循遗传程序，在一个狭窄的发育窗口内进入滞育。为了更好地理解这一决定，进行了为期5周的实验室实验，评估了食物数量和类型对脂肪积累、生物量和基因表达的影响。flemingericopepodite阶段CV。在喂食的个体中，脂质积累的初始速率在第5周减慢，因为一部分CVs开始蜕皮成虫。虽然在第1周至第3周期间，所有喂食个体的基因表达变化与发育计划一致，但CV阶段的持续时间是可变的。未喂食的个体最初保持了脂质储存，这表明生理适应是为了保存能量。与野外采集的个体基因表达谱的比较表明，对环境中的资源有类似的反应。
The high-latitude copepodNeocalanus flemingeriexploits the spring phytoplankton bloom to accumulate lipids for survival during food-limited periods and to fuel reproduction. At some point during development, lipid-accumulation ends and pre-adults molt into adults, descend to depth and enter a state of dormancy termed "diapause." How and when they determine to make this transition is still unresolved. According to one hypothesis, the trigger is their attaining a threshold amount of "lipid fullness." Alternatively, they might follow a genetic program, entering diapause within a narrow developmental window. To better understand the decision, a 5-week laboratory experiment was conducted to assess the effect of food quantity and type on lipid accumulation, biomass and gene expression inN. flemingericopepodite stage CV. In fed individuals, the initial rate of lipid accumulation slowed by week 5, as a portion of CVs began to molt into adults. While changes in gene expression common to all fed individuals between weeks 1 and 3 were consistent with a developmental program, the duration of the CV stage was variable. Unfed individuals maintained lipid stores initially, suggesting physiological acclimatization to conserve energy. A comparison with gene expression profiles of field-collected individuals suggests similar responses to resources in the environment.